Description

5B-Pregnan-3A-Ol-20-One-17,21,21,21-D4 is a high-purity, deuterium-labeled steroid derivative, specifically a tetradeuterated analog of pregnanolone. This compound is of significant value as a stable isotope-labeled internal standard for precise quantitative analysis in mass spectrometry. It is primarily needed by pharmaceutical research laboratories, contract research organizations (CROs), and analytical testing facilities engaged in method development, pharmacokinetic studies, and metabolomic profiling of related steroid hormones.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of endogenous pregnanolone and related neurosteroids in complex biological matrices like plasma, serum, and cerebrospinal fluid.
• Pharmaceutical R&D: Used in drug metabolism and pharmacokinetics (DMPK) studies to track the absorption, distribution, metabolism, and excretion of steroid-based drug candidates.
• Clinical Diagnostics: Supports the development of robust assays for measuring steroid hormone levels in clinical settings, aiding in endocrine disorder diagnosis.
• Metabolomics Research: Enables precise tracking and comparative analysis of steroid metabolic pathways in research studies.
• Reference Standard: Serves as a certified reference material (CRM) for calibrating analytical instruments and validating testing methods.
• Biochemical Research: Utilized in studies investigating the biosynthesis, regulation, and function of neuroactive steroids.

Basic Information

Product Name	5B-Pregnan-3A-Ol-20-One-17,21,21,21-D4
CAS No.	96737-93-8
Molecular Formula	C21H30D4O2
Molecular Weight	318.51 g/mol (approx.)
Synonyms	17,21,21,21-D4-Pregnanolone; Tetradeuterated Pregnanolone; 5β-Pregnan-3α-ol-20-one-d4; (3α,5β)-3-Hydroxypregnan-20-one-17,21,21,21-d4; 3α-Hydroxy-5β-pregnan-20-one-d4; Allopregnanolone-d4; 5β-Pregnan-3α-ol-20-one-d4; Deuterium Labeled Pregnanolone
EINECS	Contact for details

Quality Control

Every batch of 5B-Pregnan-3A-Ol-20-One-17,21,21,21-D4 is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity suitable for sensitive analytical applications. Comprehensive testing includes assay, isotopic enrichment, and identification by spectroscopic methods. A detailed Certificate of Analysis (COA) providing batch-specific data is available upon request to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.