Description

n-4-Azido-2-Nitrophenylpyridoxyl-5-Phosphate is a specialized biochemical reagent featuring a photoreactive azido group and a phosphate moiety. This compound is valued for its role as a cross-linking agent and a photoaffinity label in advanced biochemical research and proteomic studies. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in enzyme mechanism studies, protein labeling, and the development of novel diagnostic assays.

Application

• Photoaffinity Labeling: A critical tool for identifying and studying protein-ligand interactions, receptor binding sites, and enzyme active sites.
• Bioconjugation & Cross-linking: Used to create covalent bonds between biomolecules under controlled UV light exposure for constructing stable molecular complexes.
• Proteomics Research: Enables the tagging and isolation of specific proteins or protein complexes for mass spectrometry analysis and functional studies.
• Enzyme Mechanism Studies: Serves as an active site-directed probe to investigate catalytic mechanisms and cofactor binding in pyridoxal phosphate-dependent enzymes.
• Diagnostic Assay Development: Employed in the research phase for developing sensitive immunoassays or biosensors requiring stable, covalent attachment of detection molecules.
• Chemical Biology Tool: Facilitates the study of metabolic pathways and the spatial organization of biomolecules within cells.

Basic Information

Product Name	n-4-Azido-2-Nitrophenylpyridoxyl-5-Phosphate
CAS No.	95973-32-3
Molecular Formula	C18H15N6O8P
Molecular Weight	474.32 g/mol
Synonyms	ANP-PLP; 4-Azido-2-nitrophenyl pyridoxyl-5'-phosphate; N-4-Azido-2-nitrophenyl-5'-pyridoxylphosphate; 5'-O-Phosphono-5'-deoxy-pyridoxal 4-azido-2-nitrophenylhydrazone; Photoaffinity Labeled Pyridoxal Phosphate; PLP-ANP; Pyridoxal 5'-phosphate 4-azido-2-nitrophenylhydrazone
EINECS	Contact for details

Quality Control

Our n-4-Azido-2-Nitrophenylpyridoxyl-5-Phosphate is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and related substances is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.