Description

2-Nitrophenol-3,4,5,6-D4 is a deuterium-labeled isotopologue of 2-nitrophenol, a key compound in analytical chemistry and life science research. This stable isotope-labeled standard is essential for ensuring accuracy and reliability in quantitative mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. It is primarily used by researchers and analytical laboratories in pharmaceutical development, environmental analysis, and metabolic studies where precise internal standardization is required.

Application

• Internal Standard for Quantitative Analysis: Used as a deuterated internal standard in LC-MS/MS and GC-MS for the accurate quantification of 2-nitrophenol and related phenolic compounds in complex matrices.
• Metabolic Pathway Tracing: Employed in pharmacokinetic and drug metabolism studies to trace and quantify metabolites, leveraging the isotopic mass shift for clear detection.
• Environmental Monitoring: Serves as a reference standard in the analysis of nitrophenol pollutants in water, soil, and air samples for environmental safety assessments.
• Pharmaceutical Impurity Profiling: Critical for method development and validation in the identification and quantification of process-related impurities and degradants in active pharmaceutical ingredients (APIs).
• NMR Spectroscopy Solvent & Reagent: Used as a deuterated reagent or in deuterated solvents for NMR studies to provide spectra without interfering proton signals.
• Chemical Synthesis Research: Acts as a labeled building block in organic synthesis for preparing more complex deuterated molecules for research purposes.

Basic Information

Product Name	2-Nitrophenol-3,4,5,6-D4
CAS No.	93951-78-1
Molecular Formula	C6HD4NO3
Molecular Weight	142.12 g/mol (approx.)
Synonyms	2-Nitrophenol-d4; Deuterated 2-Nitrophenol; 2-NP-d4; o-Nitrophenol-3,4,5,6-d4; 2-Hydroxynitrobenzene-d4; (3,4,5,6-2H4)-2-Nitrophenol; 2-Nitro[3,4,5,6-2H4]phenol; NSC 403914-d4
EINECS	Contact for details

Quality Control

Every batch of 2-Nitrophenol-3,4,5,6-D4 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing performed using advanced techniques including NMR, MS, and HPLC. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (HPLC), isotopic enrichment (D%), and other critical parameters to meet the stringent requirements of research and analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container sealed to prevent moisture absorption and contamination.

Specification

Item	Specification
Appearance	Yellow to brown crystalline powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (D%)	≥ 98.0 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.