Description

Acetylcholine-1,1,2,2-D4 Bromide is a deuterium-labeled analog of the essential neurotransmitter acetylcholine, specifically designed for advanced research and analytical applications. This stable isotope-labeled compound is critical for precise quantification and mechanistic studies in neuropharmacology and biochemistry, offering unmatched specificity and reliability. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and analytical laboratories engaged in mass spectrometry, metabolic pathway tracing, and receptor binding assays.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical quantitative reference in LC-MS and GC-MS analysis of acetylcholine and related metabolites in biological matrices.
• Pharmacokinetic & Metabolic Studies: Enables precise tracking of absorption, distribution, metabolism, and excretion (ADME) of cholinergic compounds in vivo.
• Neurochemical Research: Used in receptor binding assays and enzyme activity studies (e.g., acetylcholinesterase) to investigate cholinergic system function and dysfunction.
• Biomarker Discovery & Validation: Facilitates the accurate measurement of acetylcholine levels as a potential biomarker in neurological and cardiovascular research.
• Drug Development & Quality Control: Supports the development and analytical validation of new cholinergic drugs in pharmaceutical manufacturing.

Basic Information

Product Name	Acetylcholine-1,1,2,2-D4 Bromide
CAS No.	93449-31-1
Molecular Formula	C7D4H12BrNO2
Molecular Weight	230.12 g/mol
Synonyms	(2-Acetoxyethyl)trimethylammonium-1,1,2,2-d4 Bromide; Deuterated Acetylcholine Bromide; ACh-d4 Bromide; 2-(Acetyloxy)-N,N,N-trimethylethanaminium-1,1,2,2-d4 Bromide; Acetylcholine Bromide-d4; (2-Acetyloxyethyl)trimethylammonium-d4 Bromide; Acetylcholine-d4 Bromide; ACh Bromide-d4
EINECS	Contact for details

Quality Control

Every batch of Acetylcholine-1,1,2,2-D4 Bromide is manufactured and tested under strict quality management systems to ensure the highest standards of isotopic purity and chemical integrity. Our products undergo rigorous quality testing, including NMR and mass spectrometry, to ensure compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and analytical results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.