Description

Cholesterol-D6 is a stable isotope-labeled analog of cholesterol, where six hydrogen atoms are replaced by deuterium. This compound is essential as an internal standard in quantitative mass spectrometry (MS) and liquid chromatography-mass spectrometry (LC-MS) analyses, providing unmatched accuracy for the measurement of endogenous cholesterol levels. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and life science sectors, enabling precise metabolic studies and biomarker validation.

Application

• Internal Standard for LC-MS/MS quantification of cholesterol in biological matrices (plasma, serum, tissues).
• Metabolic pathway tracing and stable isotope dilution analysis in biochemical research.
• Quality control and method development in clinical diagnostic laboratories.
• Pharmaceutical research for studying cholesterol absorption, synthesis, and drug interactions.
• Reference material for the calibration of analytical instruments.
• Use in lipidomics studies to investigate lipid metabolism and related disorders.

Basic Information

Product Name	Cholesterol-D6
CAS No.	92543-08-3
Molecular Formula	C27H40D6O
Molecular Weight	392.69 g/mol
Synonyms	Cholesterol-d6; (3β)-Cholest-5-en-3-ol-d6; Deuterated Cholesterol; 26,26,26,27,27,27-Hexadeuterocholesterol; 5-Cholesten-3β-ol-d6; Cholesterol (26,26,26,27,27,27-d6); Isotopic Labeled Cholesterol
EINECS	Contact for details

Quality Control

Our Cholesterol-D6 is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC, NMR, and MS, to ensure high chemical and isotopic purity. Certificates of Analysis (COA) detailing batch-specific results for identity, purity, and isotopic enrichment are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 99% (by HPLC)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.