Description

Cholesterol-[2,2,3,4,4,6-D6] (Certimass Solution) is a stable isotope-labeled internal standard, specifically deuterated cholesterol, essential for precise quantitative analysis in mass spectrometry. This high-purity standard is critical for ensuring analytical accuracy and method validation in complex biological matrices. It is primarily required by pharmaceutical, clinical research, and analytical laboratories for applications in lipidomics, metabolomics, and pharmacokinetic studies.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of endogenous cholesterol and related sterols in biological samples (e.g., plasma, serum, tissues).
• Clinical Diagnostics & Research: Used in method development and validation for assays measuring cholesterol levels, supporting research into cardiovascular disease, metabolic disorders, and lipid metabolism.
• Pharmaceutical Development: Employed in pharmacokinetic (PK) and bioanalytical studies to track drug-lipid interactions and assess the biodistribution of lipid-based therapeutics.
• Lipidomics & Metabolomics: Enables precise profiling and relative quantification of sterols and other lipids within complex metabolic pathways.
• Food & Nutritional Science: Applied in the analysis of cholesterol content and sterol composition in food products and dietary studies.
• Quality Control & Method Validation: Provides a reliable reference for calibrating instruments and validating analytical methods in compliance with GLP and ISO 17025 standards.

Basic Information

Item	Detail
Product Name	Cholesterol-[2,2,3,4,4,6-D6] (Certimass Solution)
CAS No.	92543-07-2
Molecular Formula	C27H40D6O
Molecular Weight	392.68 g/mol
Synonyms	Cholesterol D6; Deuterated Cholesterol; [2,2,3,4,4,6-D6]-Cholesterol; 5-Cholesten-3β-ol-d6; (3β)-Cholest-5-en-3-ol-d6; Cholesterol (2,2,3,4,4,6-hexadeuterio); Isotopic Internal Standard Cholesterol-d6; Certimass™ Cholesterol-d6 Solution
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-[2,2,3,4,4,6-D6] (Certimass Solution) is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity to meet the exacting demands of quantitative analysis. A comprehensive Certificate of Analysis (COA), detailing assay, isotopic enrichment, and solvent specifications, is provided with each shipment to ensure traceability and compliance with your research or quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at the recommended temperature as specified on the label or COA (typically 2-8°C or as required for the specific solution format). Keep the container in a dry, well-ventilated place.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (NMR/MS)	Conforms to structure
Assay (HPLC)	≥ 98% (by HPLC area)
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95%
Solvent	Certified (e.g., Methanol, Chloroform, Toluene)
Concentration	Typically 0.1-1.0 mg/mL (as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.