Description

Cholesterol Esterase CAS NO 9026-00-0 is a hydrolytic enzyme that catalyzes the cleavage of fatty acid esters of cholesterol, a critical reaction in lipid metabolism. This specificity makes it an indispensable tool for research, diagnostics, and industrial biocatalysis. It is primarily utilized by the pharmaceutical, diagnostic, and food science industries for applications ranging from cholesterol assay kits to the synthesis of fine chemicals.

Application

• Clinical Diagnostics: A key component in enzymatic assay kits for the quantitative determination of total and free cholesterol in serum, plasma, and other biological fluids.
• Biochemical Research: Used in lipid metabolism studies, membrane biology research, and as a model enzyme for investigating esterase kinetics and mechanisms.
• Pharmaceutical Development: Employed in the synthesis of steroid derivatives and as a biocatalyst in the production of chiral intermediates for drug development.
• Food & Beverage Analysis: Applied in the enzymatic analysis of cholesterol content in food products, dairy, and edible oils for nutritional labeling and quality control.
• Biotechnology: Utilized in biocatalytic processes for the modification of lipids and sterols to produce high-value compounds.

Basic Information

Product Name	Cholesterol Esterase
CAS No.	9026-00-0
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source (typically ~60-70 kDa)
Synonyms	Sterol Esterase; Cholesterol Ester Hydrolase; CEH; Sterol Ester Hydrolase; Cholesteryl Esterase; Pancreatic Cholesterol Esterase; Liver Cholesterol Esterase; Microbial Cholesterol Esterase
EINECS	232-827-7

Quality Control

Our Cholesterol Esterase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing activity (U/mg), protein content, and purity is provided with every shipment to guarantee reliable and reproducible results for our customers.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Specific Activity	≥ 50 U/mg protein (Contact for specific grade)
Protein Content (Biuret)	≥ 90% of total
Identification (IR / Activity)	Conforms
Solubility	Clear, slightly opalescent solution in buffer
pH (1% solution)	6.0 - 8.0
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.