Description

Iodonitrotetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its primary value lies in its ability to form a highly colored, water-insoluble formazan dye upon reduction, making it indispensable for visualizing metabolic activity and enzymatic reactions. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors, particularly for applications like cell viability testing (MTT assay) and microbiology.

Application

• Cell Viability and Proliferation Assays (MTT Assay): A key reagent for quantifying mitochondrial activity in live cells, critical for drug screening and cytotoxicity studies.
• Microbiological Testing: Used as an indicator for bacterial dehydrogenase activity, aiding in microbial identification and sterility testing.
• Enzyme Activity Staining: Employed in histochemistry and gel electrophoresis (e.g., for dehydrogenase enzymes) to localize enzymatic activity in tissues or polyacrylamide gels.
• Biochemical Research: Serves as an electron acceptor in numerous redox reactions, facilitating the study of metabolic pathways.
• Diagnostic Kits: Incorporated into commercial diagnostic kits for clinical microbiology and cellular analysis.
• Seed Viability Testing: Applied in agricultural science to assess seed germination potential and vigor.

Basic Information

Product Name	Iodonitrotetrazolium Chloride
CAS No.	146-68-9
Molecular Formula	C19H13ClIN5O2
Molecular Weight	505.69 g/mol
Synonyms	INT; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; Iodonitrotetrazolium; p-Iodonitrotetrazolium Violet; Tetrazolium Violet; INT Chloride; 4-Iodo-2-nitrophenyl-5-phenyl-3-tetrazolium chloride
EINECS	205-671-5

Quality Control

Our Iodonitrotetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity, identity, and performance consistency suitable for sensitive research and diagnostic applications. A detailed Certificate of Analysis (COA) providing specifications for assay, impurities, and performance in standard assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in water, ethanol; practically insoluble in ether
Performance (MTT Assay)	Meets reference standard activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.