Description

3,5-Dinitrosalicylic Acid is a specialized nitroaromatic compound widely recognized as a key reagent in analytical biochemistry. Its primary commercial value lies in its role as the active component in the DNS reagent, a fundamental tool for the quantitative determination of reducing sugars. This makes it an essential chemical for industries and research institutions focused on carbohydrate analysis, fermentation monitoring, and enzymatic activity assays. High-purity grades are critical for ensuring the accuracy and reproducibility of these sensitive analytical methods.

Application

• DNS Reagent for Sugar Analysis: The principal application is in the preparation of 3,5-dinitrosalicylic acid (DNS) reagent for the colorimetric quantification of reducing sugars (e.g., glucose, maltose) in solution.
• Biochemical Research & Enzyme Assays: Used to measure the activity of carbohydrases like amylase, cellulase, and invertase by detecting the release of reducing sugars from substrates.
• Fermentation Process Monitoring: Employed in biofuel, pharmaceutical, and food industries to track sugar consumption and product formation during microbial fermentation processes.
• Food & Beverage Quality Control: Applied in testing for sugar content in products like fruit juices, honey, syrups, and starch hydrolysates.
• Pharmaceutical Development: Utilized in research for analyzing polysaccharide-based drugs and excipients.
• Academic & Teaching Laboratories: A standard reagent in university labs for practical biochemistry and analytical chemistry courses.
• Textile & Paper Industry: Potential use in process control for enzymatic treatments of cellulose materials.

Basic Information

Product Name	3,5-Dinitrosalicylic Acid
CAS No.	609-99-4
Molecular Formula	C7H4N2O7
Molecular Weight	228.12 g/mol
Synonyms	3,5-Dinitro-2-hydroxybenzoic Acid; DNSA; 2-Hydroxy-3,5-dinitrobenzoic Acid; 3,5-Dinitrosalicylsäure (German); Acide 3,5-dinitrosalicylique (French); DNSS; DNS Reagent; Salicylic acid, 3,5-dinitro-
EINECS	210-202-6

Quality Control

Our 3,5-Dinitrosalicylic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for sensitive analytical applications. Key parameters such as Assay (Purity), Melting Point, and Absorbance Characteristics are verified. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and performance consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed to prevent absorption of atmospheric moisture, which can affect purity and performance. Keep away from heat, sparks, and open flame.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	172°C - 177°C
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Absorbance (in solution)	Conforms to standard
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.