Description

2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Mercaptan is a specialized fluorescent labeling reagent designed for the covalent modification of biomolecules and surfaces. This compound is valued for its ability to introduce a highly fluorescent fluorescein moiety via a thiol-reactive maleimide or iodoacetamide group, enabling sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and materials science sectors for applications requiring precise, stable fluorescent tagging.

Application

• Bioconjugation and Probe Synthesis: Used to label proteins, antibodies, peptides, and oligonucleotides with a fluorescein tag for fluorescence microscopy, flow cytometry (FACS), and immunoassays (ELISA).
• Diagnostic Assay Development: Serves as a key component in developing fluorescent diagnostic kits and biosensors for clinical and research use.
• Surface Functionalization: Employed to create fluorescently labeled surfaces for cell adhesion studies, microarray technology, and sensor chips.
• Drug Discovery & High-Throughput Screening (HTS): Utilized in labeling target molecules to monitor binding interactions and enzymatic activity in screening assays.
• Polymer and Nanomaterial Science: Acts as a fluorescent modifier for polymers, dendrimers, and nanoparticles to track distribution and cellular uptake.

Basic Information

Product Name	2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Mercaptan
CAS No.	887355-27-3
Molecular Formula	C24H17NO6S
Molecular Weight	447.46 g/mol
Synonyms	5-Fluoresceinylamino-2-oxoethyl mercaptan; 2-[(5-Fluoresceinyl)Aminocarbonyl]Ethanethiol; Fluorescein-5-maleimide precursor thiol; 5-(2-Mercaptoacetamido)fluorescein; Fluorescein C2 maleimide thiol adduct; Thiol-reactive fluorescein derivative; 5-[(2-Sulfanylethyl)carbamoyl]fluorescein
EINECS	Contact for details

Quality Control

Our 2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Mercaptan is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Under these conditions, the product is stable for extended periods. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.