Description

2-Thiobarbituric Acid is a heterocyclic organic compound that serves as a critical reagent in analytical and biochemical research. Its primary value lies in its ability to form colored adducts with malondialdehyde (MDA), a key biomarker for lipid peroxidation and oxidative stress. This compound is essential for researchers and quality control laboratories in the pharmaceutical, food science, and biomedical sectors who require precise measurement of oxidative damage and product stability.

Application

• Lipid Peroxidation Assay (TBARS Test): The principal application is as the core reagent in the Thiobarbituric Acid Reactive Substances (TBARS) assay for quantifying malondialdehyde, a marker of oxidative stress in biological samples, food products, and pharmaceuticals.
• Food Quality & Rancidity Testing: Used to assess the oxidative stability and shelf-life of edible oils, fats, meat, and other lipid-containing food products by measuring secondary oxidation products.
• Pharmaceutical Research: Employed in drug development and toxicology studies to evaluate the oxidative damage induced by compounds or to assess the efficacy of antioxidant drugs and formulations.
• Clinical & Diagnostic Research: Facilitates the study of oxidative stress in various disease models, including cardiovascular, neurodegenerative, and metabolic disorders.
• Cosmetics & Personal Care: Applied in stability testing of creams, lotions, and other products containing unsaturated lipids to prevent rancidity and ensure product integrity.
• Material Science: Used to study the oxidative degradation of polymers, lubricants, and other industrial materials containing susceptible organic components.

Basic Information

Product Name	2-Thiobarbituric Acid
CAS No.	504-17-6
Molecular Formula	C4H4N2O2S
Molecular Weight	144.15 g/mol
Synonyms	4,6-Dihydroxy-2-mercaptopyrimidine; Thiobarbituric Acid; TBA; 2-Thiobarbituric Acid; 2-TBA; 4,6-Dihydroxy-2-pyrimidinethiol; 2-Thiobarbituric acid (TBA); Malondialdehyde (MDA) assay reagent
EINECS	207-984-8

Quality Control

Our 2-Thiobarbituric Acid is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing to ensure high purity, consistency, and performance in sensitive assays like the TBARS test. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay purity, identification, and impurity profiles. Our quality commitment supports research reproducibility and compliance with Good Laboratory Practice (GLP) standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). The compound is light-sensitive and should be handled under appropriate conditions to prevent degradation. Keep the container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	235°C - 245°C (dec.)
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Suitability for TBARS Assay	Passes test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.