Description

Biotin-Peg5-Sh CAS NO 866935-65-1 is a heterobifunctional polyethylene glycol (PEG) linker featuring a biotin group on one terminus and a thiol-reactive maleimide group on the other. This compound is essential for creating stable, site-specific conjugates between biomolecules and surfaces, enabling advanced research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, targeted drug delivery, and the development of biosensors and diagnostic assays.

Application

• Conjugation of peptides, antibodies, or proteins to streptavidin/avidin-coated surfaces for ELISA, microarray, and biosensor platforms.
• Development of antibody-drug conjugates (ADCs) and other targeted therapeutic constructs via thiol-maleimide chemistry.
• Site-specific labeling of cysteine-containing proteins for structural biology studies, including X-ray crystallography and cryo-EM.
• Functionalization of nanoparticles, quantum dots, or other nanomaterials for in vivo imaging and targeted delivery.
• Immobilization of biomolecules on SPR (Surface Plasmon Resonance) chips and other bioanalytical surfaces.
• Preparation of affinity purification resins and chromatography media.
• As a critical reagent in the synthesis of complex biotinylated probes for pull-down assays and proteomics research.

Basic Information

Product Name	Biotin-Peg5-Sh
CAS No.	866935-65-1
Molecular Formula	C33H58N4O11S
Molecular Weight	718.9 g/mol
Synonyms	Biotin-PEG5-Maleimide; Biotin-PEG5-MAL; Maleimide-PEG5-Biotin; 2-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]ethyl 3-Maleimidopropanoate; Biotinylated PEG Maleimide; Biotin-(PEG)5-Maleimide; Biotin-PEG5-MAL
EINECS	Contact for details

Quality Control

Our Biotin-Peg5-Sh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. Under these conditions, the product is typically stable for at least 24 months.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.