Description

2‐Nitrobenzoic‐D4 Acid is a deuterated isotopologue of 2-nitrobenzoic acid, where four hydrogen atoms are replaced with deuterium. This stable isotope-labeled compound is essential for creating internal standards in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical measurements. It is a critical material for researchers and quality control laboratories in the pharmaceutical, agrochemical, and environmental testing industries who require precise quantification of target analytes.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in bioanalytical method development and validation for pharmacokinetic studies.
• Metabolite Tracing: Employed in drug metabolism and disposition (DMPK) studies to track and quantify metabolites using stable isotope dilution techniques.
• Environmental Analysis: Serves as a labeled standard for the accurate detection and measurement of nitroaromatic compounds or related degradation products in complex environmental matrices.
• Pharmaceutical Impurity Profiling: Facilitates the precise quantification of process-related impurities or degradation products in active pharmaceutical ingredients (APIs).
• Agrochemical Research: Applied in the analysis of pesticide residues and their environmental fate using isotope-labeled internal standards.
• Forensic Toxicology: Used in confirmatory testing protocols to ensure accurate and legally defensible results in substance analysis.

Basic Information

Product Name	2‐Nitrobenzoic‐D4 Acid
CAS No.	855997-63-6
Molecular Formula	C7D4H3NO4
Molecular Weight	171.14 g/mol
Synonyms	2-Nitrobenzoic-2,3,5,6-d4 Acid; Deuterated 2-Nitrobenzoic Acid; o-Nitrobenzoic Acid-d4; 2-Nitrobenzoic Acid (phenyl-d4); Benzoic-2,3,5,6-d4 Acid, 2-nitro-; 2-Nitrobenzoic Acid (Ring-d4); 2-NBD-d4; o-Nitrobenzoic-d4 Acid
EINECS	Contact for details

Quality Control

Our 2‐Nitrobenzoic‐D4 Acid is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment (typically ≥98 atom % D) and chemical purity (typically ≥95%). A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the results for identity, assay, and purity. We support compliance with cGMP and ISO 9001 standards for research and quality control applications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated area. Keep away from heat and incompatible materials.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.