Description

1,4-Dithiothreitol-1,1,2,3,4,4-D6 is a deuterated isotopologue of the classic reducing agent 1,4-dithiothreitol (DTT), specifically designed for applications requiring stable isotope labeling. This compound is critical for research where precise tracking, quantification, or elimination of background interference is necessary, particularly in mass spectrometry and nuclear magnetic resonance (NMR) studies. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and analytical service providers specializing in proteomics, structural biology, and metabolic pathway analysis.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a quantitative internal standard in LC-MS and GC-MS analyses for the accurate measurement of endogenous DTT or related thiols in complex biological matrices.
• NMR Spectroscopy Studies: Used as a deuterated tracer or solvent in NMR experiments to study protein disulfide bond reduction mechanisms and dynamics without proton signal interference.
• Proteomics and Protein Chemistry: Employed in the reduction of disulfide bonds in proteins and peptides for structural analysis, where isotope labeling helps differentiate experimental from native species.
• Metabolic Pathway Tracing: Acts as a labeled reagent in biochemical studies to trace the fate of reducing equivalents or thiol-disulfide exchange reactions in metabolic networks.
• Pharmaceutical Degradation Studies: Utilized in forced degradation studies and stability testing of biopharmaceuticals to understand reduction-related degradation pathways with high specificity.
• High-Purity Chemical Synthesis: Functions as a specialized, isotopically labeled reducing agent in the synthesis of deuterated compounds for research and development.

Basic Information

Product Name	1,4-Dithiothreitol-1,1,2,3,4,4-D6
CAS No.	850153-85-4
Molecular Formula	C4H4D6O2S2
Molecular Weight	158.28 g/mol (for D6 isotopologue)
Synonyms	(2S,3S)-1,4-Dimercapto-2,3-butanediol-1,1,2,3,4,4-d6; DTT-d6; Deuterated DTT; 1,4-Dithiothreitol-d6; meso-1,4-Dimercapto-2,3-butanediol-d6; (R*,R*)-1,4-Dimercapto-2,3-butanediol-d6; Cleland's Reagent-d6; DL-Dithiothreitol-d6
EINECS	Contact for details

Quality Control

Our deuterated chemicals are produced and controlled under stringent quality systems to ensure high isotopic purity and chemical integrity. Each batch is analyzed by advanced spectroscopic and chromatographic methods, including NMR and HPLC-MS, to confirm deuterium enrichment and chemical purity. Certificates of Analysis (COA) are provided, detailing specifications for isotopic purity (e.g., D-enrichment >98 atom % D), chemical purity, and relevant impurities. We support compliance standards relevant to research and analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation. This compound is hygroscopic (moisture-sensitive) and easily oxidized; therefore, containers should be sealed immediately after use and desiccated if necessary. For long-term stability, storage at -20°C is recommended.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Isotopic Purity (Atom % D)	≥ 98% (by NMR or MS)
Chemical Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.