Description

2-Cyanoethyl N,n-Diisopropylchlorophosphoramidite is a highly reactive phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the efficient, high-yield formation of internucleotide linkages, which is essential for producing DNA and RNA sequences. This compound is indispensable for research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the stepwise assembly of DNA and RNA strands on automated synthesizers.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and CRISPR-based therapies.
• Diagnostic Probe Synthesis: Manufacture of labeled and unlabeled DNA probes for PCR, microarrays, and in situ hybridization.
• Research-Grade Oligo Production: Synthesis of primers, linkers, and gene fragments for molecular biology and genetic research.
• Antisense Drug Development: Critical raw material in the R&D and scale-up phases of novel nucleic acid therapeutics.
• Synthesis of Modified Oligonucleotides: Used in the introduction of backbone or terminal modifications for enhanced stability and specificity.

Basic Information

Item	Detail
Product Name	2-Cyanoethyl N,n-Diisopropylchlorophosphoramidite
CAS No.	89992-70-1
Molecular Formula	C10H20ClN2OP
Molecular Weight	250.70 g/mol
Synonyms	2-Cyanoethyl N,N-Diisopropylchlorophosphoramidite; CE Phosphoramidite; Diisopropylamino-2-cyanoethoxyphosphine chloride; Chloro(2-cyanoethoxy)(diisopropylamino)phosphine; N,N-Diisopropyl-2-cyanoethylphosphoramidochloridite; Amidite C; Phosphoramidite monomer; DNA Synthesis Reagent
EINECS	Contact for details

Quality Control

Our 2-Cyanoethyl N,n-Diisopropylchlorophosphoramidite is manufactured under strict quality systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including NMR (31P and 1H) and HPLC purity analysis, to confirm identity, potency, and the absence of critical impurities like hydrolyzed species. A comprehensive Certificate of Analysis (COA) is provided with every shipment, detailing all relevant specifications to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (argon or nitrogen) at 2-8°C in a dry, cool place. Due to its moisture-sensitive and easily oxidized nature, the container should only be opened in a controlled, dry environment and resealed immediately after use.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
31P NMR Purity	≥ 98.0%
Water Content (KF)	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.