Description

L-Leucine-2-D1 is a stable isotope-labeled analog of the essential branched-chain amino acid L-Leucine, specifically deuterated at the 2-position. This precise labeling makes it an indispensable tool for advanced research and analytical applications where molecular tracking and quantification are critical. It is primarily utilized by researchers and manufacturers in the pharmaceutical, metabolic, and analytical chemistry sectors for applications such as mass spectrometry, metabolic pathway studies, and the development of internal standards.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative internal standard in LC-MS/MS and GC-MS analyses for accurate measurement of endogenous L-Leucine levels in biological samples.
• Metabolic Research: Employed in tracer studies to investigate protein synthesis, turnover, and branched-chain amino acid metabolism in vivo and in vitro.
• Pharmaceutical Development: Serves as a labeled precursor or intermediate in the synthesis of deuterated drug candidates for pharmacokinetic and metabolic stability studies.
• Nutritional Science: Used in research to study leucine's role in muscle protein synthesis, signaling pathways (e.g., mTOR), and overall metabolic health.
• Clinical Diagnostics: Facilitates the development of precise diagnostic assays for inborn errors of metabolism related to branched-chain amino acid catabolism.
• Proteomics & Stable Isotope Labeling: Incorporated into SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and other proteomic workflows for quantitative protein analysis.

Basic Information

Product Name	L-Leucine-2-D1
CAS No.	89836-93-1
Molecular Formula	C6H12DNO2
Molecular Weight	132.18 g/mol
Synonyms	(2S)-2-Amino-4-methyl-2-deuteriopentanoic acid; (S)-2-Amino-4-methyl-2-deuteriopentanoic acid; L-Leucine-2-d1; L-Leucine-d1 (2-position); Leucine-2-d1; (S)-2-Deuterioleucine; Deuterated L-Leucine; [2-2H]-L-Leucine
EINECS	Contact for details

Quality Control

Our L-Leucine-2-D1 is produced under controlled conditions to ensure high chemical and isotopic purity, meeting the stringent requirements of research and analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D%), chemical purity (by HPLC), chiral purity, and residual solvent content. We adhere to relevant quality management standards to ensure supply reliability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (D%)	≥ 98.0 atom % D
Optical Rotation	[α]D20: +14.5° to +16.5° (c=2 in 6N HCl)
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.