Description

L-Leucine-5,5,5-D3 is a stable isotope-labeled analog of the essential amino acid L-leucine, where three hydrogen atoms are replaced by deuterium at the 5,5,5 positions. This compound is critical for advanced research and development, providing a non-radioactive tracer for precise metabolic and pharmacokinetic studies. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and analytical service providers engaged in mass spectrometry, proteomics, and metabolic pathway analysis.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference standard in LC-MS and GC-MS analysis for accurate measurement of endogenous L-leucine levels in biological samples.
• Metabolic Research: Serves as a tracer to study protein synthesis, turnover, and amino acid metabolism in cell cultures, animal models, and human clinical trials.
• Proteomics & Biomarker Discovery: Enables precise relative and absolute quantification in stable isotope labeling by amino acids in cell culture (SILAC) and other proteomic workflows.
• Pharmaceutical Development: Employed in ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the fate of drug compounds and their metabolites.
• Nutritional Science Research: Investigates leucine's role in muscle protein synthesis, metabolic regulation, and its impact on health and disease states.
• Diagnostic Assay Development: Used in the calibration and validation of clinical diagnostic kits and assays measuring amino acid profiles.

Basic Information

Product Name	L-Leucine-5,5,5-D3
CAS No.	87828-86-2
Molecular Formula	C6H10D3NO2
Molecular Weight	135.19 g/mol
Synonyms	(2S)-2-Amino-4-methyl-5,5,5-trideuteriopentanoic acid; L-Leucine-d3; (S)-2-Amino-4-methyl-5,5,5-trideuteriopentanoic acid; Leucine-5,5,5-d3; (S)-Leucine-5,5,5-d3; Deuterated L-Leucine; L-Leucine (5,5,5-D3); 5,5,5-Trideutero-L-leucine
EINECS	Contact for details

Quality Control

Our L-Leucine-5,5,5-D3 is manufactured under strict quality systems to ensure high chemical purity and isotopic enrichment. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as chemical purity, isotopic purity (atom % D), chiral purity, and residual solvent content. We support research and regulatory needs with full traceability and documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to maintain stability and prevent absorption of moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0% (by MS)
Chiral Purity ([α]D)	≥ 99.0% ee
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.