Description

2,7-Bis(2-Carboxyethyl)-5(6)-Carboxyfluorescein is a highly versatile, polycarboxylated derivative of fluorescein, designed for advanced molecular conjugation and labeling applications. Its multiple carboxylate groups provide superior water solubility and enable stable covalent attachment to biomolecules, making it an essential tool for creating bright, pH-insensitive fluorescent probes. This compound is primarily utilized by researchers and manufacturers in the life sciences and diagnostics industries for developing high-performance fluorescent assays, cell-permeant indicators, and next-generation bioimaging reagents.

Application

• Fluorescent Probe Synthesis: Acts as a key precursor for synthesizing advanced fluorescent dyes and indicators, particularly cell-permeant acetoxymethyl (AM) ester derivatives for intracellular labeling.
• Bioconjugation & Labeling: Its multiple carboxylic acid groups facilitate efficient covalent coupling to proteins, antibodies, peptides, and oligonucleotides via standard amine-reactive chemistry (e.g., EDC/NHS).
• Intracellular pH & Ion Indicators: Used in the development of rationetric and intensity-based fluorescent sensors for monitoring physiological parameters like pH and metal ions within live cells.
• Diagnostic Assay Development: Serves as a critical fluorescent tag in the manufacture of immunoassays (ELISA, lateral flow), fluorescence in situ hybridization (FISH) probes, and other clinical diagnostic kits.
• Flow Cytometry & Microscopy: Enables the creation of bright, photostable fluorescent conjugates for cell sorting, analysis in flow cytometry, and high-resolution cellular imaging.
• Polymer & Material Science: Can be incorporated into hydrogels, nanoparticles, and polymeric materials to create smart, fluorescently-tagged biomaterials for research and controlled release applications.

Basic Information

Product Name	2,7-Bis(2-Carboxyethyl)-5(6)-Carboxyfluorescein
CAS No.	85138-49-4
Molecular Formula	C27H20O11
Molecular Weight	520.44 g/mol
Synonyms	BCECF; BCECF acid; 2',7'-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein; 5(6)-Carboxy-2',7'-bis(carboxyethyl)fluorescein; BCECF, Free Acid; Fluorescein derivative BCECF; 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein; 2',7'-Bis(carboxyethyl)-5(6)-carboxyfluorescein
EINECS	Contact for details

Quality Control

Our 2,7-Bis(2-Carboxyethyl)-5(6)-Carboxyfluorescein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A detailed Certificate of Analysis (COA) documenting purity, identity, and relevant specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under an inert atmosphere to maintain optimal stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.