Description

Ethyl Acetoacetate (1,2,3,4-13C4) is a stable isotope-labeled analog of ethyl acetoacetate, where four carbon atoms are enriched with the carbon-13 isotope. This compound is essential for advanced analytical and research applications requiring precise molecular tracking and quantification. It is primarily utilized by researchers and analytical laboratories in the fields of pharmaceutical development, metabolic studies, and environmental analysis. The product is supplied with a high level of isotopic enrichment and chemical purity to ensure reliable and reproducible experimental results.

Application

• As an internal standard for the accurate quantification of ethyl acetoacetate and related metabolites in complex biological matrices using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS).
• A key precursor or intermediate in the synthesis of carbon-13 labeled pharmaceuticals and fine chemicals for drug metabolism and pharmacokinetics (DMPK) studies.
• Used in metabolic pathway tracing and flux analysis to understand biochemical processes in plant, microbial, and mammalian systems.
• Employed in nuclear magnetic resonance (NMR) spectroscopy as a probe or reference compound due to its distinct isotopic signature.
• Application in environmental fate studies to track the degradation and transformation of chemical compounds.
• Serves as a building block in organic synthesis for creating complex molecules with specific isotopic labels for research purposes.

Basic Information

Product Name	Ethyl Acetoacetate (1,2,3,4-13C4)
CAS No.	84508-55-4
Molecular Formula	13C4H8O3
Molecular Weight	132.10 (for 13C4 labeled form)
Synonyms	Ethyl 3-oxobutanoate-1,2,3,4-13C4; Acetoacetic Acid Ethyl Ester-13C4; 3-Oxobutanoic Acid Ethyl Ester-13C4; EAA-13C4; Ethyl Acetoacetate-13C4; Diacetic Ester-13C4; Ethyl 3-Oxobutyrate-13C4; Ethyl Acetylacetate-13C4
EINECS	Contact for details

Quality Control

Our Ethyl Acetoacetate (1,2,3,4-13C4) is manufactured under controlled conditions to ensure exceptional isotopic and chemical purity. Each batch undergoes rigorous analysis, including NMR for isotopic enrichment verification and GC or HPLC for chemical purity assessment. We provide a comprehensive Certificate of Analysis (COA) detailing isotopic purity (atom % 13C), chemical assay, and impurity profile, ensuring traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, well-ventilated place at room temperature (15-25°C). Due to its highly volatile nature, ensure containers are securely sealed when not in use and stored away from heat and ignition sources.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Isotopic Purity (Atom % 13C)	≥ 99% (on designated positions)
Chemical Purity (GC/HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.