Description

Lithocholic-2,2,4,4-D4 Acid is a high-purity, deuterium-labeled analog of lithocholic acid, a primary bile acid. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring precise and accurate analytical measurements. It is primarily required by research institutions, pharmaceutical companies, and CROs engaged in metabolomics, pharmacokinetic studies, and biomarker discovery related to bile acid metabolism and liver function.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the accurate quantification of endogenous lithocholic acid and related bile acids in biological matrices.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) of bile acid-based therapeutics.
• Clinical Diagnostics & Biomarker Research: Enables precise measurement of bile acid profiles in serum, plasma, or urine for research into cholestatic liver diseases, gut dysbiosis, and metabolic disorders.
• Stable Isotope Dilution Assays: Provides the reference material for high-accuracy assays, minimizing matrix effects and variability in sample preparation.
• Pharmaceutical Impurity Profiling: Acts as a reference compound in the analysis and control of potential process-related impurities in drug substances.
• Nutritional and Microbiome Studies: Facilitates research on the interaction between gut microbiota and host bile acid metabolism.

Basic Information

Product Name	Lithocholic-2,2,4,4-D4 Acid
CAS No.	83701-16-0
Molecular Formula	C24H36D4O3
Molecular Weight	380.59 g/mol (for D4 isotopologue)
Synonyms	3α-Hydroxy-5β-cholan-24-oic-2,2,4,4-d4 Acid; 3α-Hydroxy-5β-cholanic-2,2,4,4-d4 Acid; LCA-d4; [2,2,4,4-D4]Lithocholic Acid; 5β-Cholan-24-oic-2,2,4,4-d4 Acid, 3α-hydroxy-; Deuterated Lithocholic Acid (2,2,4,4-D4); Lithocholic Acid-d4; 3α-Hydroxy-5β-cholanoic-2,2,4,4-d4 Acid
EINECS	Contact for details

Quality Control

Every batch of Lithocholic-2,2,4,4-D4 Acid is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity to meet the exacting demands of analytical research. Comprehensive testing includes assay by HPLC, determination of isotopic enrichment by NMR or MS, and control of related substances. A detailed Certificate of Analysis (COA) providing batch-specific data is supplied with each product.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	≥ 97%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.