Description

Cholesterol-D7 CAS NO 83199-47-7 is a stable isotope-labeled analog of cholesterol, where seven hydrogen atoms are replaced by deuterium. This high-purity internal standard is critical for ensuring the accuracy and precision of quantitative analyses in complex biological matrices. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and life science sectors requiring definitive measurements of cholesterol and its metabolites.

Application

• Quantitative Mass Spectrometry (MS): Serves as a critical internal standard for the accurate quantification of cholesterol and related sterols in LC-MS/MS and GC-MS assays.
• Clinical Research & Diagnostics: Used in method development and validation for measuring cholesterol levels in serum, plasma, and tissues to study metabolic disorders like hypercholesterolemia.
• Drug Metabolism & Pharmacokinetics (DMPK): Employed as a tracer in absorption, distribution, metabolism, and excretion (ADME) studies for lipid-based pharmaceuticals.
• Biomarker Discovery & Validation: Facilitates the precise measurement of cholesterol and its derivatives as potential biomarkers in various disease states.
• Nutritional & Food Science Analysis: Enables accurate determination of cholesterol content and sterol profiles in food products and dietary studies.
• Quality Control & Reference Material: Acts as a certified reference material (CRM) for calibrating analytical instruments and verifying assay performance in regulatory environments.

Basic Information

Product Name	Cholesterol-D7
CAS No.	83199-47-7
Molecular Formula	C27H39D7O
Molecular Weight	393.69 g/mol
Synonyms	Cholesterol-d7; (3β)-Cholest-5-en-3-ol-d7; Deuterated Cholesterol (D7); 5-Cholesten-3β-ol-d7; 3β-Hydroxy-5-cholestene-d7; [26,26,26,27,27,27-2H7]-Cholesterol; Cholesterol (26,26,26,27,27,27-d7, 3β-hydroxy-); Internal Standard for Cholesterol Analysis
EINECS	Contact for details

Quality Control

Our Cholesterol-D7 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment and chemical purity. A detailed Certificate of Analysis (COA) is provided, reporting specific parameters such as chemical purity, isotopic purity (atom % D), and residual solvent content. We support compliance with GMP/GLP guidelines for use in regulated research and production.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Purity	≥ 98.0 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.