Description

5-Methyl-D3-Uridine-6-D1 is a stable isotope-labeled nucleoside analog, specifically deuterated at multiple positions. This high-purity compound is essential for advanced research and development, providing a critical internal standard for precise quantitative analysis. It is primarily utilized by pharmaceutical researchers, analytical chemists, and diagnostic developers in the fields of metabolomics, pharmacokinetics, and biomarker discovery.

Application

• Mass Spectrometry Internal Standard: Serves as a critical reference compound for the accurate quantification of nucleoside metabolites and analogs via LC-MS/MS and GC-MS.
• Drug Metabolism & Pharmacokinetics (DMPK): Used in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) of nucleoside-based pharmaceuticals.
• Biomedical Research: Enables precise tracking and measurement of RNA turnover, methylation pathways, and cellular metabolic flux in mechanistic studies.
• Diagnostic Assay Development: Employed in the calibration and validation of high-sensitivity clinical diagnostic tests for metabolic disorders and cancer biomarkers.
• Isotope Dilution Analysis: Provides the foundation for highly accurate analytical methods in regulatory testing and quality control laboratories.

Basic Information

Product Name	5-Methyl-D3-Uridine-6-D1
CAS No.	82845-85-0
Molecular Formula	C10H11D4N2O6
Molecular Weight	265.27 g/mol
Synonyms	5-Methyluridine-d4; [6-D1, Methyl-D3]-5-Methyluridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(trideuteriomethyl)pyrimidine-2,4(1H,3H)-dione-6-d1; Uridine, 5-methyl- (methyl-D3, 6-D1)-; Stable Isotope Labeled 5-Methyluridine; Deuterated Ribothymidine
EINECS	Contact for details

Quality Control

Every batch of 5-Methyl-D3-Uridine-6-D1 is manufactured and analyzed under strict quality management systems to ensure exceptional isotopic and chemical purity. Our products undergo rigorous quality testing, including NMR and mass spectrometry for isotopic enrichment verification and HPLC for chemical purity assessment. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider keeping the product at -20°C under inert atmosphere to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D (by MS)
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.