Description

[5',5''-2H2]Uridine is a deuterium-labeled isotopologue of the essential nucleoside uridine, specifically deuterated at the 5' and 5'' positions of the ribose sugar moiety. This stable isotope labeling is critical for advanced analytical and metabolic research, providing a non-radioactive tracer with distinct mass spectral properties. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for applications in drug metabolism and pharmacokinetics (DMPK) studies, mass spectrometry-based quantification, and metabolic pathway analysis.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative internal standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the accurate measurement of endogenous uridine and related metabolites in biological matrices.
• Drug Metabolism Studies (DMPK): Used as a stable isotope-labeled tracer to investigate the absorption, distribution, metabolism, and excretion (ADME) profiles of nucleoside-based pharmaceuticals.
• Metabolic Flux Analysis: Enables the tracing and modeling of metabolic pathways in cell culture and in vivo studies, particularly in carbohydrate and nucleotide metabolism research.
• Biochemical Research: Employed in enzymatic studies to elucidate reaction mechanisms and kinetics involving uridine and its phosphorylated derivatives.
• Diagnostic Assay Development: Used in the development of high-precision clinical diagnostic kits for measuring nucleotide imbalances associated with certain metabolic disorders.

Basic Information

Product Name	[5',5''-2H2]Uridine
CAS No.	82740-98-5
Molecular Formula	C9H10D2N2O6
Molecular Weight	246.21 g/mol
Synonyms	Uridine-5',5''-d2; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl-d2)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione; Deuterated Uridine; Uridine-d2 (5',5''); 5',5''-Dideuterouridine; Stable Isotope Labeled Uridine; [5',5''-2H2]-1-β-D-Ribofuranosyluracil
EINECS	Contact for details

Quality Control

Our [5',5''-2H2]Uridine is manufactured under strict quality control protocols to ensure high chemical and isotopic purity, essential for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity (by HPLC), isotopic enrichment (by NMR and/or MS), and residual solvent content. We adhere to relevant industry standards for fine chemicals and stable isotope-labeled compounds.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at -20°C under inert atmosphere to maximize stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.