Description

16-Doxylstearoyl Maleimide is a specialized spin-labeling reagent that combines a nitroxide radical (doxyl) with a maleimide functional group via a long-chain stearoyl linker. This compound is critical for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein dynamics, conformational changes, and biomolecular interactions. It is primarily utilized by researchers and manufacturers in structural biology, biophysics, and pharmaceutical development who require precise molecular probes for advanced analytical techniques.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Covalently attaches to cysteine residues in proteins to introduce a stable nitroxide radical tag for distance measurements and dynamics studies.
• Protein Structure and Dynamics Analysis: Used to investigate protein folding, unfolding, conformational changes, and oligomerization states in solution.
• Membrane Protein Studies: The long stearoyl chain aids in labeling and studying membrane-associated proteins and their lipid interactions.
• Drug Discovery and Biologics Development: Serves as a tool to characterize protein-ligand and protein-protein interactions critical for therapeutic target validation.
• Biophysical Research Reagent: Employed in academic and industrial R&D labs for advanced spectroscopic analysis of biomacromolecules.
• Spin Probe for Polymer and Material Science: Can be used to study molecular mobility and microstructure in synthetic polymers and soft materials.

Basic Information

Product Name	16-Doxylstearoyl Maleimide
CAS No.	80263-69-0
Molecular Formula	C30H53N2O5
Molecular Weight	521.76 g/mol
Synonyms	16-DOXYL-stearic acid maleimide; N-(1-Oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-16-maleimidostearamide; 16-Maleimidostearic Acid 4-(1-Oxyl-2,2,6,6-tetramethylpiperidine) Ester; 16-Maleimidostearoyl TEMPO; 16-(Maleimidoyl)stearoyl-4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl; 16-Doxyl-stearic acid maleimide; Stearic acid, 16-(maleimidoyl)-, 4-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl) ester; Spin-labeling reagent SL-MTS
EINECS	Contact for details

Quality Control

Our 16-Doxylstearoyl Maleimide is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, essential for sensitive spectroscopic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic methods for structural confirmation. A Certificate of Analysis (COA) detailing specific results for purity, identity, and related substances is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
EPR Signal	Characteristic nitroxide radical spectrum
Solubility	Soluble in DMSO, DMF, chloroform; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.