Description

Dl-Proline-2,5,5-D3 is a deuterium-labeled isotopologue of the amino acid proline, specifically deuterated at the 2, 5, and 5 positions. This compound is essential for advanced research and development, serving as a critical internal standard and tracer in mass spectrometry and metabolic studies to ensure analytical accuracy and traceability. It is primarily utilized by pharmaceutical R&D teams, analytical laboratories, and academic researchers working in drug metabolism and pharmacokinetics (DMPK), proteomics, and stable isotope labeling.

Application

• Internal Standard for LC-MS/MS: Provides precise quantification of proline and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in stable isotope-resolved metabolomics (SIRM) to study collagen synthesis, cellular stress responses, and amino acid flux.
• Pharmaceutical DMPK Studies: Critical for investigating the absorption, distribution, metabolism, and excretion (ADME) of proline-containing drug candidates.
• Proteomics Research: Enables protein turnover studies and peptide quantification through metabolic labeling techniques.
• Biochemical Research: Serves as a tool for enzymatic studies and mechanistic investigations involving proline metabolism.
• Quality Control Reference: Acts as a certified reference material (CRM) for method validation and ensuring analytical data integrity in regulated environments.

Basic Information

Product Name	Dl-Proline-2,5,5-D3
CAS No.	784086-28-8
Molecular Formula	C₅H₆D₃NO₂
Molecular Weight	118.15 g/mol
Synonyms	DL-Proline-2,5,5-d3; (RS)-Proline-2,5,5-d3; (±)-Proline-2,5,5-d3; Pyrrolidine-2-carboxylic-2,5,5-d3 acid; 2,5,5-Trideutero-DL-proline; Deuterated DL-proline; Proline-d3; DL-Proline (2,5,5-D3)
EINECS	Contact for details

Quality Control

Our Dl-Proline-2,5,5-D3 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR (¹H, ²H) and mass spectrometry, to confirm isotopic enrichment and chemical purity. We provide comprehensive Certificates of Analysis (COA) detailing assay, isotopic purity, and impurity profiles to support your regulatory and research requirements. Our commitment ensures material traceability and consistency for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (²H NMR/MS)	≥ 98 atom % D
Chemical Purity (Assay)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.