Description

n-tert-Boc-L-Alanine-D4 is a stable isotope-labeled derivative of the amino acid L-alanine, featuring a tert-butoxycarbonyl (Boc) protecting group and deuterium substitution. This compound is a critical building block for the synthesis of isotopically labeled peptides and proteins, enabling advanced research in mass spectrometry, metabolic studies, and pharmacokinetics. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions focused on proteomics and drug discovery.

Application

• Internal Standard for LC-MS/MS: Serves as a quantitative reference standard in bioanalytical methods for accurate measurement of alanine or related peptides in complex biological matrices.
• Proteomics Research: Used in the synthesis of stable isotope-labeled peptides (SIL peptides) for mass spectrometry-based protein quantification and biomarker discovery.
• Metabolic Pathway Tracing: Employed in deuterium-labeling studies to investigate amino acid metabolism, protein turnover rates, and metabolic flux analysis.
• Pharmaceutical Development: A key intermediate in the preparation of isotopically labeled active pharmaceutical ingredients (APIs) for drug metabolism and pharmacokinetics (DMPK) studies.
• NMR Spectroscopy: Useful as a deuterated probe in nuclear magnetic resonance (NMR) spectroscopy to simplify spectra and study molecular structure and dynamics.
• Bioconjugation & Chemical Biology: Facilitates the site-specific incorporation of labels into biomolecules for tracking and imaging applications.

Basic Information

Product Name	n-tert-Boc-L-Alanine-D4
CAS No.	714964-61-1
Molecular Formula	C8H11D4NO4
Molecular Weight	191.23 g/mol
Synonyms	(2S)-2-[(tert-Butoxycarbonyl)amino]propanoic-3,3,3-d3 acid; (2S)-2-[(tert-Butoxycarbonyl)amino]-3,3,3-trideuteriopropanoic acid; Boc-L-alanine-d4; tert-Butoxycarbonyl-L-alanine-d4; N-Boc-L-alanine-d4; N-(tert-Butoxycarbonyl)-L-alanine-d4; L-Alanine-d4, N-Boc-; BOC-Ala-OH-d4
EINECS	Contact for details

Quality Control

Our n-tert-Boc-L-Alanine-D4 is manufactured under strict quality control protocols to ensure high isotopic purity and chemical integrity, suitable for sensitive analytical applications. Each batch is characterized by NMR, HPLC, and mass spectrometry to confirm chemical purity, isotopic enrichment (typically >98 atom % D), and the absence of critical impurities. Certificates of Analysis (COA) with detailed spectroscopic and chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (NMR/MS)	≥98 atom % D
Optical Rotation	[α]20/D: -25° to -30° (c=1 in EtOH)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.