Description

16Alpha-Hydroxy-17Beta-Estradiol-2,4,17-D3 is a high-purity, deuterium-labeled analog of a key estrogen metabolite, specifically designed for analytical and research applications. This compound is essential for use as an internal standard in mass spectrometry, enabling precise quantification and method validation in complex biological matrices. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers engaged in hormone analysis, metabolic studies, and bioequivalence testing.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of endogenous estradiol and its metabolites in pharmacokinetic and bioanalytical studies.
• Metabolic Pathway Research: Used as a tracer to investigate steroid hormone metabolism and biotransformation pathways in vitro and in vivo.
• Clinical Diagnostics Development: Supports the development and validation of high-sensitivity assays for hormonal disorders and endocrine function.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of related substances in drug substance and product analysis.
• Doping Control Analysis: Employed in sports anti-doping laboratories for the definitive detection of synthetic steroid abuse.
• Environmental Bio-monitoring: Facilitates the study of endocrine-disrupting chemicals and their effects on steroid hormone levels.

Basic Information

Product Name	16Alpha-Hydroxy-17Beta-Estradiol-2,4,17-D3
CAS No.	79037-36-8
Molecular Formula	C18H21D3O3
Molecular Weight	291.41 g/mol
Synonyms	16α-Hydroxyestradiol-2,4,17-d3; 16α-Hydroxy-17β-estradiol-2,4,17-d3; Estra-1,3,5(10)-triene-3,16α,17β-triol-2,4,17-d3; [2,4,17-2H3]-16α-Hydroxyestradiol; Deuterated 16α-Hydroxyestradiol; 16α-OH-E2-d3; Internal Standard for Estradiol Metabolite Analysis.
EINECS	Contact for details

Quality Control

Every batch of 16Alpha-Hydroxy-17Beta-Estradiol-2,4,17-D3 is manufactured under controlled conditions and undergoes rigorous analytical characterization to ensure identity, purity, and isotopic enrichment. Our quality assurance protocol includes NMR, HPLC, and mass spectrometric analysis. A comprehensive Certificate of Analysis (COA) detailing purity, chemical purity, isotopic purity (D-enrichment), and residual solvent levels is provided with each shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This material is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Chemical Purity (HPLC)	≥ 95.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.