Description

2-Hydroxybenzoic-3,4,5,6-D4 Acid is a deuterium-labeled isotopologue of salicylic acid, where four hydrogen atoms on the aromatic ring are replaced with deuterium. This high-purity labeled standard is critical for ensuring analytical accuracy and reliability in quantitative mass spectrometry and NMR spectroscopy. It is an essential material for researchers and quality control laboratories in the pharmaceutical, agrochemical, and environmental testing industries who require precise internal standards for method development and validation.

Application

• Internal Standard for Quantitative Analysis: Used as a stable, non-interfering internal standard in LC-MS and GC-MS for the accurate quantification of salicylic acid and related compounds in complex matrices.
• Metabolic Pathway Tracing: Enables detailed pharmacokinetic and metabolic studies in pharmaceutical research by tracking the fate of salicylic acid and its derivatives in biological systems.
• NMR Spectroscopy Solvent & Reference: Serves as a chemical shift reference or a deuterated solvent additive in nuclear magnetic resonance (NMR) experiments.
• Method Development & Validation: Critical for developing and validating robust analytical methods in compliance with regulatory guidelines (e.g., FDA, ICH) for pharmaceutical quality control.
• Environmental Analysis: Applied in trace analysis of salicylates in environmental water and soil samples to monitor pollution and degradation pathways.
• Isotope Dilution Mass Spectrometry (IDMS): Provides the highest level of measurement accuracy in definitive methods for clinical diagnostics and reference material certification.

Basic Information

Product Name	2-Hydroxybenzoic-3,4,5,6-D4 Acid
CAS No.	78646-17-0
Molecular Formula	C7H2D4O3
Molecular Weight	142.15 g/mol
Synonyms	Salicylic Acid-d4; 2-Hydroxybenzoic-d4 Acid; Deuterated Salicylic Acid; Benzoic-3,4,5,6-d4 Acid, 2-hydroxy-; [2H4]-Salicylic Acid; 3,4,5,6-Tetradeuterosalicylic Acid; o-Hydroxybenzoic-d4 Acid; 2-Hydroxy[3,4,5,6-2H4]benzoic Acid
EINECS	Contact for details

Quality Control

Our deuterated compounds are manufactured under strict quality systems to ensure high isotopic purity and chemical integrity. Each batch is characterized by NMR and mass spectrometry to confirm deuteration level and chemical purity. We provide comprehensive Certificates of Analysis (COA) detailing assay, isotopic enrichment, and impurity profiles, supporting compliance with GMP and ISO 9001 standards for research and regulatory submission.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0% (chemical purity)
Isotopic Purity (MS)	≥98 atom % D
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.