Description

3-Nitrobenzoic-D4 Acid is a deuterium-labeled derivative of 3-nitrobenzoic acid, serving as a critical internal standard and tracer in advanced analytical chemistry. Its value lies in enabling precise quantification and metabolic pathway elucidation through mass spectrometry, minimizing interference from endogenous compounds. This high-purity material is essential for researchers and quality control laboratories in the pharmaceutical, agrochemical, and environmental analysis sectors, where accurate isotope dilution methods are required.

Application

• Pharmaceutical Research & Development: Used as a stable isotope-labeled internal standard in LC-MS and GC-MS for the pharmacokinetic and metabolic studies of drug candidates and related compounds.
• Analytical Method Development: Critical for developing and validating highly accurate quantitative analytical methods in compliance with regulatory guidelines (e.g., FDA, ICH).
• Metabolomics & Biomarker Discovery: Employed as a tracer to investigate biochemical pathways and identify novel biomarkers in complex biological matrices.
• Environmental Analysis: Facilitates the precise detection and quantification of nitroaromatic compounds and their degradation products in environmental samples.
• Chemical Synthesis Intermediary: Acts as a deuterated building block for the synthesis of more complex labeled compounds for research purposes.
• Quality Control & Reference Standards: Serves as a certified reference material (CRM) to ensure the accuracy and precision of analytical instruments and procedures.

Basic Information

Product Name	3-Nitrobenzoic-D4 Acid
CAS No.	78399-78-7
Molecular Formula	C7HD4NO4
Molecular Weight	171.14 g/mol
Synonyms	3-Nitrobenzoic-2,3,5,6-d4 Acid; Benzoic-2,3,5,6-d4 Acid, 3-Nitro-; Deuterated 3-Nitrobenzoic Acid; 3-Nitrobenzoic Acid-d4; m-Nitrobenzoic Acid-d4; [2,3,5,6-2H4]-3-Nitrobenzoic Acid; 3-Nitro[2,3,5,6-2H4]benzoic Acid
EINECS	Contact for details

Quality Control

Our 3-Nitrobenzoic-D4 Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR and Mass Spectrometry, to confirm isotopic enrichment and chemical purity. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic purity, and identified impurities are provided. We support compliance with relevant pharmacopeial and research standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0% (Chemical Purity)
Isotopic Purity (NMR/MS)	≥98.0 atom % D
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.