Description

Ethyl Acetoacetate (1,3-13C2) is a stable isotope-labeled derivative of the versatile chemical intermediate ethyl acetoacetate, specifically enriched with carbon-13 at the 1 and 3 positions. This labeled compound is essential for advanced analytical and research applications where precise molecular tracking is required. It is primarily utilized by researchers and manufacturers in the pharmaceutical, agrochemical, and analytical chemistry sectors for applications such as metabolic pathway studies, internal standards for mass spectrometry, and the synthesis of isotopically labeled target molecules.

Application

• Internal Standard for Quantitative Analysis: Serves as a critical reference standard in Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) for the accurate quantification of unlabeled ethyl acetoacetate and related compounds.
• Metabolic Pathway Tracing: Used in biomedical and biochemical research to elucidate metabolic fluxes and mechanisms in vivo and in vitro through isotope labeling experiments.
• Synthesis of Labeled Compounds: Acts as a key building block for the preparation of more complex carbon-13 labeled pharmaceuticals, agrochemicals, and fine chemicals.
• NMR Spectroscopy Studies: Employed as a probe in Nuclear Magnetic Resonance (NMR) spectroscopy to study molecular structure, dynamics, and reaction mechanisms due to its distinct isotopic signature.
• Pharmaceutical R&D: Facilitates drug discovery and development processes, including pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.

Basic Information

Item	Detail
Product Name	Ethyl Acetoacetate (1,3-13C2)
CAS No.	77504-73-5
Molecular Formula	C6H1013C2O3
Molecular Weight	~132.13 g/mol (for 13C2 labeled form)
Synonyms	Ethyl 3-oxobutanoate-1,3-13C2; Acetoacetic Acid Ethyl Ester-1,3-13C2; 3-Oxobutanoic Acid Ethyl Ester-1,3-13C2; EAA-13C2; Diethyl Ketone-3-carboxylate-1,3-13C2; Ethyl Acetoacetate-1,3-13C2; 1,3-13C2-Ethyl Acetoacetate
EINECS	Contact for details

Quality Control

Our Ethyl Acetoacetate (1,3-13C2) is produced under stringent conditions to ensure high isotopic and chemical purity, meeting the exacting requirements of research and analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and identity confirmation. We adhere to rigorous quality testing protocols to guarantee consistency and reliability for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). Keep away from strong bases and incompatible materials such as strong oxidizing agents. The container should be kept tightly sealed to minimize exposure to moisture and air.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Isotopic Enrichment (13C)	≥ 99 atom % 13C (at positions 1 & 3)
Chemical Purity (GC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.