Description

L-Valine-2-D1 is a stable isotope-labeled analog of the essential amino acid L-Valine, specifically deuterated at the 2-position. This compound is a critical tool in advanced research and analytical chemistry, enabling precise metabolic and pharmacokinetic studies through mass spectrometry and NMR spectroscopy. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers for tracer studies, internal standard applications, and proteomics research.

Application

• Mass Spectrometry Internal Standard: Serves as a critical quantitative reference standard in LC-MS and GC-MS analysis for accurate measurement of endogenous L-Valine levels in biological samples.
• Metabolic Pathway Tracing: Used in deuterium-labeling studies to investigate amino acid metabolism, protein synthesis rates, and metabolic flux in cell cultures, animal models, and clinical research.
• NMR Spectroscopy: Provides a non-radioactive tracer for structural elucidation and dynamic studies of biomolecules and metabolic processes.
• Pharmaceutical Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies to track the fate of valine-containing drug candidates or co-administered nutrients.
• Proteomics & Stable Isotope Labeling by Amino acids in Cell culture (SILAC): Facilitates quantitative proteomic analysis to measure differential protein expression.
• Clinical Diagnostics: Potential use in developing advanced assays for inborn errors of metabolism related to branched-chain amino acid pathways.

Basic Information

Product Name	L-Valine-2-D1
CAS No.	77257-03-5
Molecular Formula	C5H10DNO2
Molecular Weight	118.16 g/mol
Synonyms	(2S)-2-Amino-3-methyl-2-deuteriobutanoic acid; (S)-2-Amino-3-methyl-2-deuteriobutanoic acid; L-Valine-2-d; Valine-2-d1; Deuterated L-Valine; L-Valine (2-d1); [2-D]L-Valine; (S)-2-Deutero-3-methylbutyric acid
EINECS	Contact for details

Quality Control

Every batch of L-Valine-2-D1 is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Our products undergo rigorous quality testing, including isotopic enrichment analysis by NMR and MS, chiral purity verification, and chemical purity assessment by HPLC. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic purity, optical rotation, and related substances are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Optical Rotation	+26.5° to +29.0° (c=1 in 6N HCl)
Chiral Purity (HPLC)	≥ 99.0% L-isomer
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.