Description

Polyphyllin B (Rg) is a bioactive steroidal saponin, a key marker compound derived from the traditional medicinal plant *Paris polyphylla*. This high-purity reference standard is critical for ensuring the quality, efficacy, and consistency of botanical extracts and finished products. It is primarily required by analytical laboratories, pharmaceutical R&D teams, and nutraceutical manufacturers focused on standardization and advanced phytochemical research.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quantitative and qualitative analysis of herbal drugs and formulations containing *Paris polyphylla*.
• Bioactivity Research: Employed in pharmacological studies to investigate its potential anti-tumor, anti-inflammatory, and hemostatic properties in vitro and in vivo.
• Herbal Extract Standardization: Serves as a primary marker for the quality control and standardization of commercial botanical extracts to ensure batch-to-batch consistency and potency.
• Analytical Method Development: Essential for developing and validating HPLC, UPLC, and LC-MS/MS methods in quality control laboratories.
• Academic Research: Used in universities and research institutes for phytochemical, metabolic, and biosynthetic pathway studies.
• Dietary Supplement Authentication: Acts as a key identifier to authenticate raw materials and finished nutraceutical products, preventing adulteration.

Basic Information

Item	Detail
Product Name	Polyphyllin B (Rg)
CAS No.	76296-73-6
Molecular Formula	C51H82O21
Molecular Weight	1031.18 g/mol
Synonyms	Polyphyllin B; Polyphyllin Rg; Paris saponin Rg; (25R)-Spirost-5-en-3β-yl O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside; Pennogenin 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside; Diosgenin 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Every batch of Polyphyllin B (Rg) is produced and analyzed under strict quality management protocols. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles, typically determined by HPLC-DAD/ELSD and confirmed by NMR and MS. Our quality system is designed to meet the stringent requirements for analytical reference standards and supports compliance in research and regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Loss on Drying	≤2.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.