Description

Copper 2,2'-Bicinchoninate is a coordination complex formed between copper ions and the bicinchoninic acid (BCA) ligand. This compound is of significant commercial and research importance as a key component in the widely used BCA protein assay, a colorimetric method for the quantitative determination of total protein concentration. It is essential for laboratories and production facilities in the biotechnology, pharmaceutical, diagnostic, and life science research sectors requiring precise and reliable protein analysis.

Application

• Primary Reagent in BCA Protein Assay Kits: The core chromogenic agent for colorimetric protein quantification in solution.
• Biopharmaceutical Manufacturing: Used in process development and quality control (QC) to monitor protein expression, purification yield, and final product concentration.
• Academic & Industrial Research: Enables protein concentration measurement in a wide range of biochemical and cell biology experiments.
• Diagnostic Kit Formulation: Incorporated into in-vitro diagnostic (IVD) reagents for clinical chemistry analyzers.
• Food & Beverage Analysis: Applied in QC labs for determining protein content in nutritional products and raw materials.
• Contract Research Organizations (CROs): A standard reagent for high-throughput screening and bioanalytical services.

Basic Information

Product Name	Copper 2,2'-Bicinchoninate
CAS No.	76109-99-4
Molecular Formula	C20H12CuN2O4
Molecular Weight	407.87 g/mol
Synonyms	Copper(II) Bicinchoninate; Cupric Bicinchoninate; BCA-Copper Complex; BCA-Cu; 2,2'-Bicinchoninic Acid Copper Salt; 4,4'-Dicarboxy-2,2'-biquinoline Copper Complex; Copper 2,2'-Biquinoline-4,4'-dicarboxylate
EINECS	Contact for details

Quality Control

Our Copper 2,2'-Bicinchoninate is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance in sensitive analytical applications. Each lot is tested to meet rigorous specifications for identity, purity, and chromogenic activity. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and performance in a standard BCA assay is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry environment. The material is light-sensitive; prolonged exposure to light should be avoided to maintain stability and assay performance.

Specification

Item	Specification
Appearance	Dark green to purple solid or solution
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Chromogenic Activity	Meets performance standard vs. reference
Loss on Drying	≤ 2.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.