Description

Dl-Phenyl-D5-Alanine-3,3-D2 is a stable isotope-labeled analog of the essential amino acid phenylalanine, specifically deuterated at multiple positions. This high-purity compound is critical for applications requiring precise internal standards and metabolic tracers in advanced analytical and research workflows. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and contract research organizations (CROs) specializing in mass spectrometry, metabolic pathway analysis, and pharmacokinetic studies.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical quantitative reference in LC-MS and GC-MS analyses for accurate measurement of phenylalanine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in stable isotope labeling studies to elucidate biochemical pathways, protein synthesis rates, and metabolic flux in cell cultures, tissues, and whole organisms.
• Pharmaceutical Research & Development: Enables precise pharmacokinetic (PK) and pharmacodynamic (PD) studies for drug candidates, facilitating the understanding of absorption, distribution, metabolism, and excretion (ADME).
• Clinical Diagnostics & Biomarker Discovery: Assists in the development and validation of assays for inborn errors of metabolism (e.g., Phenylketonuria - PKU) and other metabolic disorders.
• Proteomics & Protein Turnover Studies: Employed in metabolic labeling (SILAC, Stable Isotope Labeling by Amino acids in Cell culture) to study protein dynamics, expression, and degradation.
• NMR Spectroscopy: Acts as a deuterated standard or solvent for nuclear magnetic resonance spectroscopy, helping to simplify spectra and assign molecular structures.

Basic Information

Product Name	Dl-Phenyl-D5-Alanine-3,3-D2
CAS No.	74228-83-4
Molecular Formula	C9D7H4NO2
Molecular Weight	171.22 g/mol (deuterated form)
Synonyms	DL-Phenylalanine-2,3,3,4,5,5,6-d7; (RS)-2-Amino-3-(2,3,4,5,6-pentadeuteriophenyl)propionic-3,3-d2 acid; Deuterated DL-Phenylalanine; Phenylalanine-d7; DL-α-Amino-β-(pentadeuteriophenyl)propionic-3,3-d2 acid; (rac)-Phenylalanine-d7; 3-Phenyl-d5-alanine-3,3-d2
EINECS	Contact for details

Quality Control

Our Dl-Phenyl-D5-Alanine-3,3-D2 is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity, meeting the rigorous demands of analytical and research applications. Each batch is characterized by advanced analytical techniques including NMR, MS, and HPLC to confirm isotopic enrichment, chemical identity, and purity. Certificates of Analysis (COA) detailing isotopic purity (atom % D), chemical purity, and analytical results are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant or inert atmosphere conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0% (by MS)
Chemical Purity	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.