Description

Phenobarbital-D5 CAS NO 72793-46-5 is a stable isotope-labeled analog of the barbiturate drug phenobarbital, where five hydrogen atoms are replaced by deuterium. This compound is essential as a high-purity internal standard for quantitative mass spectrometry, ensuring analytical accuracy and method validation. It is primarily required by pharmaceutical research laboratories, forensic toxicology units, and contract research organizations (CROs) for drug metabolism studies, pharmacokinetic analysis, and compliance testing.

Application

• Internal Standard for LC-MS/MS: Critical for the precise quantification of phenobarbital in biological matrices (plasma, serum, urine) during bioanalytical method development and validation.
• Pharmaceutical R&D: Used in drug metabolism and pharmacokinetics (DMPK) studies to track the absorption, distribution, metabolism, and excretion (ADME) of the parent drug.
• Forensic and Clinical Toxicology: Enables accurate measurement of phenobarbital levels for therapeutic drug monitoring (TDM), overdose cases, and forensic investigations.
• Reference Standard: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring the reliability of testing protocols in quality control laboratories.
• Isotope Dilution Mass Spectrometry (IDMS): Employed in this gold-standard technique to achieve the highest possible accuracy in quantitative analysis, minimizing matrix effects.

Basic Information

Product Name	Phenobarbital-D5
CAS No.	72793-46-5
Molecular Formula	C12H7D5N2O3
Molecular Weight	236.27 g/mol
Synonyms	5-Ethyl-5-phenyl-d5-2,4,6(1H,3H,5H)-pyrimidinetrione; Phenobarbital-d5; 5-Ethyl-5-(phenyl-d5)-barbituric Acid; Luminal-d5; Phenobarbital (ethyl-d5, phenyl-d5); Deuterated Phenobarbital; Phenobarbital Internal Standard
EINECS	Contact for details

Quality Control

Our Phenobarbital-D5 is manufactured under strict quality systems to meet the exacting requirements of analytical reference standards. Each batch is characterized using advanced spectroscopic and chromatographic techniques, including NMR and High-Resolution Mass Spectrometry (HRMS), to confirm isotopic purity and chemical identity. A comprehensive Certificate of Analysis (COA) is provided, detailing assay (typically ≥98% chemical and isotopic purity), chromatographic purity, and residual solvent content, ensuring compliance with ISO 17034 guidelines for reference material producers.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a desiccated environment to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Purity	≥98 atom % D
Chemical Purity (HPLC)	≥99.0%
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Complies with ICH Q3C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.