Description

4-Cholesten-3-One-2,2,4,6,6-D5 is a deuterium-labeled analog of cholestenone, a key intermediate in steroid biosynthesis and metabolism research. This high-purity, isotopically labeled compound is essential for applications requiring precise internal standards and metabolic pathway tracing. It is primarily utilized by research institutions, pharmaceutical R&D laboratories, and analytical service providers engaged in advanced mass spectrometry, metabolic studies, and stable isotope labeling.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical quantitative reference standard in LC-MS and GC-MS analysis of sterols and related metabolites.
• Metabolic Pathway Tracing: Used in biochemical research to elucidate cholesterol biosynthesis, catabolism, and steroid hormone production pathways.
• Pharmaceutical R&D: Employed in the development and validation of analytical methods for drug metabolism and pharmacokinetics (DMPK) studies involving steroidal compounds.
• Biomarker Research: Facilitates the accurate quantification of cholestenone and its derivatives in biological samples for clinical and diagnostic research.
• Stable Isotope Labeling Studies: Acts as a tracer in mechanistic studies to investigate enzyme kinetics and metabolic flux.
• Quality Control Reference Material: Used by manufacturers of analytical standards and reagents to ensure batch-to-batch consistency and accuracy.

Basic Information

Product Name	4-Cholesten-3-One-2,2,4,6,6-D5
CAS No.	72560-60-2
Molecular Formula	C27H39D5O
Molecular Weight	389.66 g/mol (approximate)
Synonyms	Cholest-4-en-3-one-2,2,4,6,6-d5; 5-Cholesten-3-one-2,2,4,6,6-d5; Deuterated Cholestenone; [2,2,4,6,6-2H5]-Cholest-4-en-3-one; 4-Cholesten-3-one-d5; Cholestenone-d5; 3-Oxocholest-4-ene-2,2,4,6,6-d5
EINECS	Contact for details

Quality Control

Our 4-Cholesten-3-One-2,2,4,6,6-D5 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment and chemical purity, ensuring it meets the exacting standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic purity, and analytical results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.