Description

[2H4]-Testosterone is a stable isotope-labeled analog of the primary male sex hormone, testosterone, where four hydrogen atoms are replaced by deuterium. This high-purity reference standard is essential for precise quantitative analysis in research and development, enabling accurate measurement and validation of testosterone levels. It is a critical material for analytical laboratories in the pharmaceutical, clinical diagnostics, and anti-doping control sectors.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a crucial internal standard for the accurate quantification of endogenous testosterone in biological matrices via LC-MS/MS and GC-MS.
• Clinical Diagnostics and Research: Used in the development and validation of assays for hormonal disorder diagnosis, endocrine research, and metabolic studies.
• Pharmaceutical Development: Employed in pharmacokinetic (PK) and bioavailability studies of testosterone-based therapeutics and related compounds.
• Anti-Doping Analysis: A vital reference material for World Anti-Doping Agency (WADA)-accredited laboratories to detect and confirm the abuse of exogenous testosterone in athletes.
• Metabolic Pathway Tracing: Utilized in stable isotope tracer studies to investigate androgen metabolism and biosynthesis pathways.
• Method Development and Validation: Essential for developing, calibrating, and verifying analytical methods to ensure accuracy, precision, and regulatory compliance.

Basic Information

Product Name	[2H4]-Testosterone
CAS No.	638163-36-7
Molecular Formula	C19H242H4O2
Molecular Weight	292.45 g/mol
Synonyms	Testosterone-d4; [17β-Hydroxyandrost-4-en-3-one]-d4; Deuterated Testosterone; Androst-4-en-17β-ol-3-one-d4; 17β-Hydroxy-4-androsten-3-one-d4; 4-Deuterotestosterone; Isotopic Testosterone Standard
EINECS	Contact for details

Quality Control

Our [2H4]-Testosterone is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR, MS, and HPLC, to ensure high chemical purity and isotopic enrichment. Certificates of Analysis (COA) detailing purity, isotopic abundance, and solvent residues are provided to guarantee traceability and compliance with research and regulatory standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (deuterium incorporation)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.