Description

6-Hydroxy Melatonin-D4 is a stable isotope-labeled analog of the primary metabolite of melatonin, where four hydrogen atoms are replaced by deuterium. This compound is essential as a high-purity internal standard for the accurate quantification of 6-hydroxymelatonin in biological matrices via mass spectrometry. It is a critical reagent for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and life science sectors requiring precise analytical measurements.

Application

• LC-MS/MS Internal Standard: Primary use as a deuterated internal standard for the precise quantification of 6-hydroxymelatonin in pharmacokinetic and metabolic studies.
• Biomarker Analysis: Enables accurate measurement of 6-sulfatoxymelatonin (aMT6s) levels in urine, a key biomarker for circadian rhythm research and sleep disorder diagnostics.
• Drug Metabolism & Pharmacokinetics (DMPK): Used in ADME (Absorption, Distribution, Metabolism, and Excretion) studies to track the metabolic pathway of melatonin and related analogs.
• Clinical Research: Supports studies investigating melatonin's role in sleep regulation, jet lag, and seasonal affective disorder (SAD) through reliable metabolite tracking.
• Reference Standard: Serves as a certified reference material (CRM) for method development and validation in analytical laboratories.
• Pharmaceutical Quality Control: Applied in the assay and impurity profiling of melatonin-based pharmaceutical formulations.

Basic Information

Product Name	6-Hydroxy Melatonin-D4
CAS No.	69533-61-5
Molecular Formula	C₁₃H₁₂D₄N₂O₂
Molecular Weight	236.30 g/mol
Synonyms	6-Hydroxymelatonin-d4; N-[2-(5-Methoxy-1H-indol-3-yl)ethyl]acetamide-d4; 6-OH-Melatonin-d4; Deuterated 6-Hydroxymelatonin; 6-Hydroxy-5-methoxy-N-acetyltryptamine-d4; Melatonin Metabolite-d4; aMT6-d4; 6-Sulfatoxymelatonin Precursor-d4
EINECS	Contact for details

Quality Control

Our 6-Hydroxy Melatonin-D4 is manufactured under strict quality systems to ensure high isotopic purity and chemical integrity, suitable for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (typically ≥98% D), chemical purity (by HPLC), and confirmation of structure. We adhere to relevant standards for high-purity reference materials, and custom synthesis to meet specific pharmacopeial or internal specifications is available.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Purity (D-enrichment)	≥98% (by MS)
Assay (HPLC)	≥95%
Chemical Purity (HPLC)	≥98%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.