Description

4'-Nitroacetanilide-2',3',5',6'-D4 is a deuterium-labeled analog of 4-nitroacetanilide, a key intermediate in organic synthesis and analytical chemistry. This high-purity, isotopically labeled compound is essential for applications requiring internal standards for mass spectrometry, enabling precise quantification and metabolic pathway tracing. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical service providers engaged in drug metabolism and pharmacokinetics (DMPK) studies, as well as advanced organic synthesis.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, non-interfering internal standard for the accurate quantification of 4-nitroacetanilide and related metabolites in complex biological matrices.
• Drug Metabolism Studies (DMPK): Used in pharmacokinetic and metabolic stability assays to track the fate of parent compounds and identify metabolites.
• Isotope Dilution Mass Spectrometry (IDMS): Enables highly accurate and precise quantitative analysis, a gold-standard method in regulatory bioanalysis.
• Mechanistic Organic Chemistry Research: Acts as a deuterated probe in reaction mechanism studies to investigate kinetic isotope effects (KIEs).
• Synthesis of Labeled Compounds: Functions as a building block for the preparation of more complex deuterium-labeled molecules for research and development.

Basic Information

Product Name	4'-Nitroacetanilide-2',3',5',6'-D4
CAS No.	68239-25-8
Molecular Formula	C8H4D4N2O3
Molecular Weight	184.18 g/mol
Synonyms	N-(4-Nitrophenyl)acetamide-d4; 4-Nitroacetanilide-d4; p-Nitroacetanilide-d4; N-Acetyl-4-nitroaniline-d4; 4'-Nitroacetanilide-2',3',5',6'-d4; Acetamide, N-(4-nitrophenyl)-2,3,5,6-d4-; Deuteronated 4-nitroacetanilide
EINECS	Contact for details

Quality Control

Every batch of 4'-Nitroacetanilide-2',3',5',6'-D4 is manufactured and tested under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, supported by comprehensive analytical data including NMR, HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) specifying purity, isotopic purity (D-enrichment), and impurity profile is provided with each shipment to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated area. Keep away from heat and incompatible materials.

Specification

Item	Specification
Appearance	Yellow to pale brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.