Description

Phenolphthaleindiphosphatepentasodiumsaltgr is a high-purity, water-soluble sodium salt derivative of phenolphthalein diphosphate, a key chromogenic substrate. This compound is essential for enzymatic assays where precise and sensitive detection of alkaline phosphatase activity is required. It is primarily utilized by diagnostic reagent manufacturers, life science research institutions, and quality control laboratories in the pharmaceutical and biotechnology sectors.

Application

• Diagnostic Reagent Manufacturing: As a critical chromogenic substrate in clinical chemistry for the detection and quantification of alkaline phosphatase (ALP) in serum and other biological fluids.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used in research and diagnostic ELISA kits for colorimetric detection, offering high sensitivity and a clear visual endpoint.
• Biochemical Research: A fundamental tool for studying enzyme kinetics, inhibition, and the function of alkaline phosphatase in various metabolic pathways.
• Pharmaceutical Quality Control: Employed in in-process testing and final product release assays to monitor enzymatic activity during biopharmaceutical production.
• Histochemistry & Cell Biology: Used as a substrate for visualizing alkaline phosphatase activity in tissue sections and cell cultures.
• Food Safety Testing: Potential application in developing rapid test kits for detecting bacterial contamination based on microbial enzyme activity.

Basic Information

Product Name	Phenolphthaleindiphosphatepentasodiumsaltgr
CAS No.	68007-90-9
Molecular Formula	C20H12Na5O16P2 (or similar, based on pentasodium salt)
Molecular Weight	Contact for details
Synonyms	Phenolphthalein Diphosphate Pentasodium Salt; Phenolphthalein Bis(phosphate) Pentasodium Salt; PPP; Phenolphthalein Diphosphate, Sodium Salt; ALP Substrate; 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone Bis(dihydrogen phosphate) Pentasodium Salt; Diagnostic Reagent Grade Phenolphthalein Diphosphate; Chromogenic Alkaline Phosphatase Substrate
EINECS	Contact for details

Quality Control

Our Phenolphthaleindiphosphatepentasodiumsaltgr is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for critical diagnostic and research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity, spectrophotometric analysis for functional performance, and stringent limits for moisture and heavy metals. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content	≤ 5.0%
Solubility (in Water)	Clear, colorless solution
Functional Performance (Enzymatic)	Conforms to reference (δA/min)
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.