Description

[2H4]-n-Acetyl-5-Hydroxytryptamine CAS NO 66521-40-2 is a stable isotope-labeled analog of N-acetylserotonin, specifically deuterated at four positions on the indole ring. This compound is an essential internal standard for the precise quantification of melatonin and related indoleamine metabolites in complex biological matrices using mass spectrometry. It is primarily utilized by analytical laboratories, pharmaceutical R&D departments, and clinical research organizations engaged in neurochemistry, chronobiology, and metabolic pathway studies.

Application

• Quantitative Mass Spectrometry: Serves as a critical internal standard for Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Mass Spectrometry (GC-MS) methods to ensure accuracy and reproducibility in bioanalysis.
• Pharmacokinetic & Metabolomic Studies: Enables precise tracking of melatonin biosynthesis, metabolism, and clearance in preclinical and clinical research settings.
• Diagnostic Assay Development: Used in the calibration and validation of commercial and in-house diagnostic kits for measuring melatonin and serotonin-related biomarkers.
• Neuroendocrine Research: Facilitates the study of the pineal gland function, circadian rhythm regulation, and sleep disorder mechanisms.
• Quality Control in Pharmaceutical Manufacturing: Employed in the analytical testing of melatonin-based pharmaceutical products and nutraceuticals to verify potency and purity.
• Forensic Toxicology: Aids in the detection and quantification of melatonin and its analogs in forensic investigations.

Basic Information

Product Name	[2H4]-n-Acetyl-5-Hydroxytryptamine
CAS No.	66521-40-2
Molecular Formula	C12H10D4N2O2
Molecular Weight	218.26 g/mol
Synonyms	N-Acetyl-5-hydroxytryptamine-d4; N-Acetylserotonin-d4; 5-Hydroxy-N-acetyltryptamine-d4; N-[2-(5-Hydroxy-1H-indol-3-yl)ethyl]acetamide-d4; Melatonin Precursor-d4; Deuterated N-Acetylserotonin; d4-NAS; NAS-d4
EINECS	Contact for details

Quality Control

Our [2H4]-n-Acetyl-5-Hydroxytryptamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment and chemical purity, ensuring it meets the exacting standards required for quantitative bioanalysis. A comprehensive Certificate of Analysis (COA) detailing isotopic purity, chemical purity, and solvent content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage, keep the primary container under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.