Description

n-Acetyl-5-Methoxytryptamine-Alpha,Alpha,Beta,β-D4 is a deuterated analog of melatonin, a key biochemical used in research and development. This stable isotope-labeled compound is essential for creating internal standards in quantitative analytical methods like LC-MS/MS, ensuring precise and accurate measurement of melatonin and its metabolites. It is primarily utilized by analytical laboratories, pharmaceutical R&D departments, and contract research organizations (CROs) focused on neurochemistry, chronobiology, and pharmacokinetic studies.

Application

• Internal Standard for Mass Spectrometry: Serves as a critical deuterated internal standard for the precise quantification of melatonin in biological matrices via LC-MS/MS and GC-MS.
• Pharmacokinetic & Metabolite Studies: Enables accurate tracking of melatonin absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Method Development & Validation: Used to develop, optimize, and validate highly sensitive and specific analytical methods in compliance with GLP guidelines.
• Biochemical Research: A tool for investigating melatonin's role in sleep regulation, antioxidant activity, and immune function without interference from endogenous compounds.
• Quality Control in Pharmaceutical Manufacturing: Supports assay and impurity profiling of melatonin-based pharmaceutical products and nutraceuticals.
• Forensic & Doping Control Analysis: Applied in specialized testing for the detection and confirmation of exogenous melatonin administration.

Basic Information

Item	Detail
Product Name	n-Acetyl-5-Methoxytryptamine-Alpha,Alpha,Beta,β-D4
CAS No.	66521-38-8
Molecular Formula	C₁₃H₁₂D₄N₂O₂
Molecular Weight	236.30 g/mol
Synonyms	Melatonin-d4; N-Acetyl-5-methoxytryptamine-d4; 5-Methoxy-N-acetyltryptamine-d4; N-[2-(5-Methoxy-1H-indol-3-yl)ethyl]acetamide-d4; Circadin-d4 (labeled analog); MT-d4; MLT-d4; Deuterated Melatonin
EINECS	Contact for details

Quality Control

Our n-Acetyl-5-Methoxytryptamine-d4 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and Mass Spectrometry, to confirm identity, chemical purity, and deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Incorporation)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.