Description

(+/-)-Cotinine-D3 CAS NO 66269-66-7 is a deuterium-labeled isotopologue of cotinine, a major metabolite of nicotine, serving as a crucial internal standard for analytical quantification. Its primary value lies in enabling highly accurate and sensitive mass spectrometric analysis by correcting for instrument variability and matrix effects. This compound is essential for researchers and quality control laboratories in the pharmaceutical, clinical research, and forensic toxicology sectors who require precise measurement of nicotine and its metabolites in biological samples.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative internal standard in liquid chromatography-tandem mass spectrometry for the analysis of cotinine and nicotine in plasma, urine, and saliva.
• Pharmacokinetic and Metabolic Studies: Facilitates research into the absorption, distribution, metabolism, and excretion (ADME) of nicotine in both clinical and pre-clinical settings.
• Biomarker Validation: Critical for developing and validating analytical methods to measure cotinine as a biomarker for tobacco smoke exposure and smoking cessation studies.
• Forensic Toxicology: Employed in forensic laboratories for the accurate determination of nicotine intake in legal and workplace testing scenarios.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring method accuracy and compliance.
• Drug Development: Supports pharmaceutical R&D for nicotine replacement therapies (NRTs) and other smoking cessation aids by providing reliable metabolic data.

Basic Information

Item	Detail
Product Name	(+/-)-Cotinine-D3
CAS No.	66269-66-7
Molecular Formula	C10H9D3N2O
Molecular Weight	179.23 g/mol
Synonyms	Cotinine-d3; (RS)-Cotinine-d3; (±)-Cotinine-d3; 5-(3-Pyridyl)-1-methyl-2-pyrrolidinone-d3; 1-Methyl-5-(3-pyridinyl)-2-pyrrolidinone-d3; Nicotine Metabolite-d3; NSC 5292-d3; Cotinine (methyl-d3)
EINECS	Contact for details

Quality Control

Our (+/-)-Cotinine-D3 is produced under strict quality management systems to ensure the high purity and isotopic enrichment required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity, isotopic purity (D-enrichment), and residual solvent content. We adhere to relevant standards for high-purity reference materials to support your GLP and ISO 17025 compliant laboratory workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term stability, consider storage at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 99.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.