Description

Nornicotine-2,4,5,6-D4 (Pyridine-D4) is a deuterium-labeled analog of the tobacco alkaloid nornicotine, specifically designed for advanced analytical and research applications. This stable isotope-labeled compound is essential for use as an internal standard in mass spectrometry, enabling precise quantification and metabolic pathway tracing in complex biological matrices. It is primarily utilized by researchers and analytical laboratories in the fields of pharmaceutical development, forensic toxicology, and environmental analysis to ensure data accuracy and reliability.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of nornicotine and related alkaloids in pharmacokinetic and bioanalytical studies.
• Metabolic Pathway Research: Used as a tracer to investigate the biotransformation and distribution of nicotine derivatives in biological systems.
• Forensic and Clinical Toxicology: Enables precise measurement of tobacco alkaloid exposure and metabolite profiling in human samples.
• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for method validation and quality control in drug development labs.
• Environmental Analysis: Assists in tracking nicotine-related pollutants and their degradation products in environmental samples.
• Isotope Dilution Mass Spectrometry (IDMS): Provides high-accuracy results in quantitative analysis where maximum precision is required.

Basic Information

Product Name	Nornicotine-2,4,5,6-D4 (Pyridine-D4)
CAS No.	66148-18-3
Molecular Formula	C9H8D4N2
Molecular Weight	162.23 g/mol (for D4 isotopologue)
Synonyms	2,4,5,6-Tetradeutero-nornicotine; Nornicotine-d4; (S)-Nornicotine-2,4,5,6-d4; 1-Methyl-2-(3-pyridyl)pyrrolidine-2,4,5,6-d4; Deuterated Nornicotine; Pyridine-d4 Nornicotine; Nornicotine (tetradeuterated); Stable Isotope Labeled Nornicotine
EINECS	Contact for details

Quality Control

Every batch of Nornicotine-2,4,5,6-D4 is produced and analyzed under strict quality management protocols to ensure the highest isotopic and chemical purity for research applications. Our quality assurance includes comprehensive testing via NMR, HPLC, and mass spectrometry to confirm isotopic enrichment, chemical identity, and purity. Certificates of Analysis (COA) detailing batch-specific results for isotopic purity (e.g., D4 ≥ 98%) and chemical assay are provided and traceable to in-house reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated place. This compound is hygroscopic (moisture-sensitive); therefore, containers must be kept tightly sealed to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D4)	≥ 98 atom % D
Chemical Purity (Titration)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.