Description

Diazepam-D5 CAS NO 65854-76-4 is a deuterium-labeled internal standard of the widely used benzodiazepine, diazepam, essential for precise quantitative analysis in research and pharmaceutical development. Its primary value lies in enabling accurate and reliable measurements in mass spectrometry-based assays, such as LC-MS/MS, by correcting for variations in sample preparation and instrument response. This high-purity reference material is critically needed by analytical laboratories, pharmaceutical R&D teams, and forensic toxicology units for method development, validation, and routine drug monitoring.

Application

• Internal Standard for Bioanalytical Assays: Used as a deuterated internal standard in the quantification of diazepam and its metabolites in biological matrices (plasma, serum, urine) via LC-MS/MS.
• Pharmaceutical Research & Development: Critical for pharmacokinetic (PK) and pharmacodynamic (PD) studies, ensuring accurate data for drug development and regulatory submissions.
• Forensic and Clinical Toxicology: Enables precise measurement of diazepam levels in forensic investigations, workplace drug testing, and therapeutic drug monitoring (TDM).
• Method Development and Validation: Serves as a key component in developing, optimizing, and validating robust analytical methods according to FDA, EMA, or other regulatory guidelines.
• Quality Control in Manufacturing: Supports impurity profiling and assay verification during the production of diazepam-based pharmaceutical products.
• Metabolite Studies: Aids in the identification and quantification of diazepam metabolites in research focused on drug metabolism and disposition.

Basic Information

Product Name	Diazepam-D5
CAS No.	65854-76-4
Molecular Formula	C16H8D5ClN2O
Molecular Weight	289.77 g/mol
Synonyms	7-Chloro-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one-d5; Deuterated Diazepam; Diazepam (phenyl-d5); Diazepam-d5 (major); Valium-d5; 2H-1,4-Benzodiazepin-2-one, 7-chloro-1-methyl-5-(phenyl-d5)-1,3-dihydro-; 5-(Phenyl-d5)-7-chloro-1-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one
EINECS	Contact for details

Quality Control

Our Diazepam-D5 is manufactured under strict quality systems to ensure the high purity and isotopic enrichment required for sensitive analytical applications. Each batch undergoes comprehensive testing, including assay by HPLC, determination of isotopic purity by mass spectrometry, and identification by IR and NMR. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent specifications and supporting your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature, typically between 15-25°C (59-77°F). Keep the container in a dry, cool, and well-ventilated area. For long-term stability, consider storage at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.