Description

Bis[N,n-Bis(Carboxymethyl)Aminomethyl]Fluorescein Tetrasodium Salt is a high-purity, water-soluble fluorescent chelating agent primarily used as a sensitive indicator for calcium ions. This compound matters for its exceptional selectivity and high quantum yield, enabling precise detection and quantification of calcium in complex matrices. It is critically needed by researchers and quality control laboratories in the life sciences, pharmaceutical development, and clinical diagnostics sectors for applications ranging from intracellular calcium imaging to assay development.

Application

• Fluorescent Calcium Indicator: For real-time visualization and measurement of intracellular calcium ion (Ca²⁺) flux in live-cell imaging and flow cytometry.
• Biochemical Assay Reagent: Used in the development of high-throughput screening (HTS) assays and enzymatic activity tests where calcium is a cofactor or product.
• Diagnostic Kit Component: Employed in clinical diagnostic kits for the quantitative analysis of calcium in biological fluids such as serum and urine.
• Research Chemical: Serves as a key tool in neuroscience, cardiology, and cell biology research to study calcium signaling pathways.
• Quality Control Testing: Applied in pharmaceutical and nutraceutical quality control to assay calcium content in raw materials and finished products.
• Environmental Analysis: Potential use in environmental monitoring for detecting calcium and other divalent cations in water samples.

Basic Information

Product Name	Bis[N,n-Bis(Carboxymethyl)Aminomethyl]Fluorescein Tetrasodium Salt
CAS No.	62698-56-0
Molecular Formula	C₃₄H₂₈N₂Na₄O₁₄
Molecular Weight	794.55 g/mol
Synonyms	Fluo-4 Tetrasodium Salt; Fluo-4, Tetrasodium Salt; Bis(2-{[bis(carboxymethyl)amino]methyl}-6-{2-[bis(carboxymethyl)amino]phenoxy}-3H-xanthen-3-ylidene)bis(methanylylidene)tetrakis(acetic acid) tetrasodium salt; Calcium Green-1; Fluorescent Calcium Indicator Fluo-4; BAPTA-based fluorescent dye; 5,5'-Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein tetrasodium salt
EINECS	Contact for details

Quality Control

Our Bis[N,n-Bis(Carboxymethyl)Aminomethyl]Fluorescein Tetrasodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional testing for fluorescence performance. Certificates of Analysis (COA) detailing purity, identity, and assay results are provided with every shipment to ensure compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be allowed to reach room temperature before opening to minimize moisture uptake. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em ~494/516 nm (with Ca²⁺)
Calcium Binding Affinity (Kd)	~345 nM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.