Description

Pregnenolone-17Alpha,21,21,21-D4 is a high-purity, deuterium-labeled analog of the key steroid hormone precursor pregnenolone. This stable isotope-labeled compound is essential for advanced analytical and research applications requiring precise quantification and metabolic pathway tracing. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers specializing in steroid analysis and biomarker discovery.

Application

• Internal Standard for Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical quantitative reference standard for the accurate measurement of endogenous pregnenolone and related steroid metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in pharmacokinetic (PK) and drug metabolism studies to track the biotransformation of steroid-based compounds.
• Biomarker Research and Validation: Enables the development and validation of highly sensitive and specific clinical assays for steroid hormone disorders.
• Pharmaceutical Reference Standard: Employed in quality control and method development for the analysis of steroid active pharmaceutical ingredients (APIs) and finished products.
• Endocrinology and Neuroscience Research: Facilitates studies on neurosteroid synthesis, function, and their role in various physiological and pathological processes.

Basic Information

Product Name	Pregnenolone-17Alpha,21,21,21-D4
CAS No.	61574-54-7
Molecular Formula	C21H28D4O2
Molecular Weight	318.50 g/mol (approx.)
Synonyms	17α-Hydroxy-21,21,21-trideuteropregnenolone; (3β)-3-Hydroxy-21,21,21-trideuteropregn-5-en-20-one; Deuterated Pregnenolone; Pregnenolone-d4; 17α-Hydroxypregnenolone-d4; 5-Pregnen-3β,17α-diol-20-one-21,21,21-d3; Pregnenolone-17α-ol-21,21,21-d3
EINECS	Contact for details

Quality Control

Every batch of Pregnenolone-17Alpha,21,21,21-D4 is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to ensure identity, purity, and isotopic enrichment meet the exacting standards required for research and analytical use. A detailed Certificate of Analysis (COA) specifying results for assay, isotopic purity, and related substances is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 99.0%
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.