Description

Cholesterol-26,26,26,27,27,27-D6 is a high-purity, stable isotope-labeled analog of cholesterol, specifically deuterated at six positions on the terminal methyl groups of the side chain. This compound is an essential internal standard and tracer in advanced analytical and metabolic research, providing unmatched accuracy for quantification and pathway elucidation. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions engaged in lipidomics, biomarker discovery, and drug metabolism studies.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the precise and accurate quantification of cholesterol and its metabolites in complex biological matrices.
• Metabolic Flux Analysis: Used as a stable isotope tracer to investigate cholesterol biosynthesis, absorption, and turnover rates in in vitro and in vivo studies.
• Clinical Diagnostics & Biomarker Research: Enables the development and validation of high-sensitivity assays for cholesterol-related biomarkers in serum, plasma, and tissues.
• Pharmaceutical Development: Facilitates ADME (Absorption, Distribution, Metabolism, Excretion) studies for drug candidates targeting lipid pathways, ensuring regulatory compliance.
• Lipidomics and Systems Biology: A key tool in comprehensive lipid profiling to understand lipid metabolism disorders, such as atherosclerosis and NASH.
• Quality Control & Method Validation: Employed in analytical laboratories to validate cholesterol assay methods, ensuring accuracy, precision, and robustness.

Basic Information

Product Name	Cholesterol-26,26,26,27,27,27-D6
CAS No.	60816-17-3
Molecular Formula	C₂₇H₄₀D₆O
Molecular Weight	392.69 g/mol
Synonyms	Cholesterol-d6 (26,26,26,27,27,27); (3β)-Cholest-5-en-3-ol-26,26,26,27,27,27-d6; Deuterated Cholesterol (6D); 5-Cholesten-3β-ol-26,26,26,27,27,27-d6; Cholesterol (26,26,26,27,27,27-hexadeuterio); Stable Isotope Labeled Cholesterol; Internal Standard Cholesterol-d6
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-26,26,26,27,27,27-D6 is manufactured and analyzed under strict quality management systems. Our products undergo rigorous quality testing, including isotopic purity and chemical purity assays, to ensure compliance with the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing batch-specific results for isotopic enrichment, chemical purity, and identity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. For short-term use, the material may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Purity	≥ 98.0 atom % D
Isotopic Enrichment	≥ 99.0% at labeled positions (by MS)
Loss on Drying	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.