Description

Hygromycin A CAS NO 6379-56-2 is an aminoglycoside antibiotic produced by *Streptomyces hygroscopicus*. This compound is valued for its potent activity against a broad spectrum of bacteria and its specific use as a selective agent in molecular biology and cell culture. It is primarily utilized by researchers and manufacturers in the life sciences sector for applications requiring the elimination of specific cell populations. Key industries include pharmaceutical R&D, biotechnology, and academic research institutions.

Application

• Selective Agent in Cell Culture: Used to select for eukaryotic cells transfected with a hygromycin resistance gene, enabling stable cell line development.
• Microbiological Research: Serves as a tool antibiotic in bacterial genetics and microbiology studies to investigate resistance mechanisms and gene function.
• Antibiotic Screening: Employed in assays to screen for novel antimicrobial compounds or to study antibiotic synergy and antagonism.
• Veterinary Research: Historically studied for potential use in animal health, though largely superseded by other agents; remains a reference compound.
• Biochemical Tool: Used to study protein synthesis inhibition, specifically targeting the translocation step on the ribosome.
• Plant Biotechnology: Applied as a selective agent in the transformation and regeneration of plant tissues.

Basic Information

Product Name	Hygromycin A
CAS No.	6379-56-2
Molecular Formula	C20H37N3O13
Molecular Weight	527.52 g/mol
Synonyms	Hygromycin A; Antibiotic XK-33-5; O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl-(1-5)-2-deoxy-N3-methyl-D-streptamine; Hygromix; XK 33-5; NSC 529634; Antibiotic 172A
EINECS	228-947-3

Quality Control

Our Hygromycin A is produced and tested under a strict quality management system to ensure high purity and batch-to-batch consistency, suitable for research and cell culture applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, potency, and microbiological testing. We adhere to relevant industry standards for fine chemicals used in biological research.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and potency. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Potency (Microbiological)	≥ 750 µg/mg
pH (1% solution)	5.0 - 7.0
Bacterial Endotoxins	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.