Description

n-Hydroxysuccinimide CAS NO 6066-82-6 is a vital chemical intermediate widely recognized for its role as an activating agent in peptide synthesis and bioconjugation chemistry. Its primary value lies in its ability to efficiently form stable amide bonds under mild conditions, which is critical for creating complex biomolecules and functionalized materials. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-purity reagents for developing APIs, antibody-drug conjugates, and biosensors.

Application

• Peptide Synthesis: As a key component for activating carboxylic acids to form NHS esters, facilitating efficient amide bond formation.
• Bioconjugation & Crosslinking: For labeling proteins, antibodies, and other biomolecules with fluorescent dyes, enzymes, or drug molecules in diagnostic and therapeutic development.
• Polymer & Material Science: Used to functionalize polymers, nanoparticles, and surfaces with reactive groups for creating advanced biomaterials and coatings.
• Antibody-Drug Conjugate (ADC) Production: A critical reagent in the linker chemistry for attaching cytotoxic payloads to monoclonal antibodies.
• Carbohydrate & Nucleic Acid Chemistry: Employed in the derivatization and modification of sugars and oligonucleotides for research applications.
• Chromatography & Affinity Purification: Used to prepare NHS-activated agarose or sepharose resins for immobilizing ligands in column purification.

Basic Information

Product Name	n-Hydroxysuccinimide
CAS No.	6066-82-6
Molecular Formula	C4H5NO3
Molecular Weight	115.09 g/mol
Synonyms	NHS; 1-Hydroxy-2,5-pyrrolidinedione; N-Hydroxysuccinimide; HOSu; N-Hydroxy-2,5-pyrrolidinedione; Succinimide, N-hydroxy-; 2,5-Pyrrolidinedione, 1-hydroxy-; N-Hydroxybutanimide
EINECS	228-001-3

Quality Control

Our n-Hydroxysuccinimide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive applications. We provide comprehensive analytical data, including HPLC purity, melting point, and residual solvent analysis. A Certificate of Analysis (COA) is supplied with each batch, and material can be produced to meet specific customer requirements or relevant standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.5%
Melting Point	95 - 100 °C
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.